Five Minute 6-plex Mouse mAb Isotyping Kit

样本 ：Mouse

标记物 ：Mouse

应用 ：Mouse

规格 ：5T/20T

产品特色

公司旗舰产品线包括免疫检查点蛋白，生物素标记蛋白，Fc受体蛋白等，基于AviTagTM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。多年来，Product Description
The Five-Minute 6-plex Mouse mAb Isotyping Kit provides a lateral flow assay for monoclonal antibody class and subclass, and light chain type determination. When a sample is loaded onto the cassette, the antibodies will form soluble complexes with pre-embedded gold conjugates. These complexes are resolved and captured by class or isotype specific antibodies impregnated at different areas of the membrane. As a result, a red line will appear at the corresponding region with isotype indicated. This kit can be used to assay for IgG1, IgG2a, IgG2b, and IgM, along with lambda and kappa light chain types. 
The tests for IgG3 and IgA are not included in this product. If you want to assay for these two isotypes, please use our 8-plex mouse mAb isotyping kit (Cat. # E001-K004).
Kit Components
The kit contains 5 (Cat. E001-K001-5) or 20 (Cat. E001-K001-20) pouches with 2 cassettes in each pouch. One cassette is for assessing IgG1, IgG2a, IgG2b, and the other one is for Kappa, Lambda and IgM. Please note that a control (“C”) line is included in both cassette.
Sample Requirements
The minimum detectable concentration is 100 ng/mL. Below is our recommendation on sample preparation.

Protocol
1.Place the cassettes on a flat surface.
2.Add 100 µL sample directly to the well and wait for 5 minutes.
3.A red line should appear on the membrane with specific isotypes indicated at the same position. A red line should also appear at the “C” position.
NOTE1:Prepare samples before removing the strips from the sealed pouch.
NOTE2:Do not read after 20 minutes.
NOTE3:The color of tissue culture medium may cause high background. To address this issue, additional 40-60 µL PBS can be added into the well to clear the background. 

Storage
Please keep the strips in their original packaging at room temperature. This product is stable for up to 18 months from the certified date. Please do NOT freeze the strips.
Cell Culture Supernatant    Purified Antibody    Ascites
1:100 dilution in PBS (optional)    0.5-2 µg/mL in PBS    1:10,000 in PBS *
* Normally, ascites fluid contains multiple Igs. The kit can only be used to determine the relative distribution of the main isotypes.不断研究与发展，以满足客户不断提高的期望与需求，为医疗行业的发展贡献我们的一份力量。