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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum, plasma(heparin, EDTA) and urine.
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0483 |
|---|---|
| 产品名称 | Mouse OPN ELISA Kit |
| 产品描述 | For quantitative detection of mouse OPN in cell culture supernates, serum, plasma(heparin, EDTA) and urine. |
| 免疫原 | NSO, L17-N294 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | SPP1 |
| 基因全名 | secreted phosphoprotein 1 |
| Uniprot ID | P10923 |
| 指标别名 | BNSP|Bone sialoprotein 1|Bone sialoprotein I|BSPI|Early T lymphocyte activation 1|ETA 1|MGC110940|Nephropontin|OPN|Osteopontin|OSTP_HUMAN|Secreted phosphoprotein 1|Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1)|Secreted phosphoprotein 1 (osteopontin bone sialoprotein)|SPP-1|SPP1|Urinary stone protein|Uropontin |
| 背景资料 | Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0483-CAP | 96-well plate precoated with anti- mouse OPN antibody | 1 |
| EK0483-ST | Lyophilized recombinant mouse OPN standard | 10ng/tubex2 |
| EK0483-DA | Biotinylated anti- mouse OPN antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 306 | 1986 | 5401 | 308 | 2000 | 4959 |
| Standard deviation | 14.68 | 117.17 | 405.07 | 15.09 | 142 | 446.31 |
| CV(%) | 4.8% | 5.9% | 7.5% | 4.9% | 7.1% | 9% |
Mouse OPN ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.046 | 0.116 | 0.198 | 0.305 | 0.483 | 0.818 | 1.503 | 2.032 |
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