Rat NGFβ ELISA Kit

价格： ￥2280

品牌：BOSTER 品牌认证

货号：EK0471

产品详情

产品概况

货号	EK0471
产品名称	Rat NGFβ ELISA Kit
产品描述	For quantitative detection of in cell culture supernates and serum.
免疫原	NSO, S122-G241
交叉反应	There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.
敏感性	<1pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	NGF
基因全名	nerve growth factor (beta polypeptide)
Uniprot ID	P25427
指标别名	Beta nerve growth factor\|Beta NGF\|HSAN5\|MGC161426\|MGC161428\|Nerve growth factor (beta polypeptide)\|Nerve growth factor beta polypeptide\|Nerve growth factor beta subunit\|NGFB\|NID67
背景资料	Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0471-CAP	96-well plate precoated with anti- rat NGF antibody	1
EK0471-ST	Lyophilized recombinant rat NGF standard	10ng/tubex2
EK0471-DA	Biotinylated anti- rat NGF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1. 96孔读板仪器。

2. 自动洗板机.

3. 移液器和枪头。建议使用多通道移液器/排枪。

4. Eppendorf 管。

5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

● 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

● 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	191	480	29	201	491
Standard deviation	1.84	8.02	32.64	1.62	11.65	35.84
CV(%)	5.6%	4.2%	6.8%	5.6%	5.8%	7.3%