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库存 :发货周期:现货
供应商 :博士德生物
应用 :cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
规格 :96T
产品概况
货号 | EK0469 |
---|---|
产品名称 | Human NGF/NGF beta ELISA Kit |
产品描述 | For quantitative detection of human NGF in cell culture supernates and serum. |
免疫原 | NSO, S122-A241 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | NGF |
基因全名 | nerve growth factor (beta polypeptide) |
Uniprot ID | P01138 |
指标别名 | Beta nerve growth factor|Beta NGF|HSAN5|MGC161426|MGC161428|Nerve growth factor (beta polypeptide)|Nerve growth factor beta polypeptide|Nerve growth factor beta subunit|NGFB|NID67 |
背景资料 | Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0469-CAP | 96-well plate precoated with anti- human NGF antibody | 1 |
EK0469-ST | Lyophilized recombinant human NGF standard | 10ng/tubex2 |
EK0469-DA | Biotinylated anti- human NGF antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1. 96孔读板仪器。
2. 自动洗板机.
3. 移液器和枪头。建议使用多通道移液器/排枪。
4. Eppendorf 管。
5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 216 | 468 | 30 | 203 | 429 |
Standard deviation | 1.38 | 14.47 | 20.59 | 1.8 | 13.8 | 22.3 |
CV(%) | 4.2% | 6.7% | 4.4% | 6% | 6.8% | 5.2% |
Human NGF/NGF beta ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.091 | 0.127 | 0.157 | 0.226 | 0.342 | 0.682 | 1.074 | 1.987 |
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入驻年限:17年