phospho-NIPA(Ser354) antibody

供应商 ：上海康朗生物科技有限公司

库存 ：大量

目录编号 ：kl-8364R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：12个月

抗体英文名 ：phospho-NIPA(Ser354) antibody

抗体名 ：磷酸化间变性淋巴瘤激酶核相互作用伴侣蛋白抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,

免疫原 ：KLH conjugated synthesised phosphopeptide derived from human NIPA around the phosphorylation site of Ser354:TR(p-S)WD

亚型 ：IgG

形态 ：冻干粉或液体

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:50-200

浓度 ：1mg/ml

保存条件 ：-20 °C

规格 ：100ul

phospho-NIPA(Ser354) antibody

中文名称	磷酸化间变性淋巴瘤激酶核相互作用伴侣蛋白抗体
别    名	NIPA (phospho S354); NIPA (phospho Ser354); p-NIPA (S354); p-NIPA (Ser354); hNIPA; Nuclear interacting partner of ALK; Nuclear interacting partner of anaplastic lymphoma kinase; ZC3HC1; Zinc finger C3HC type containing 1; NIPA_HUMAN.
规格价格	100ul/1580元 购买        大包装/询价
说 明 书	100ul
产品类型	磷酸化抗体
研究领域	肿瘤  细胞生物  免疫学  染色质和核信号  信号转导  激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	55kDa
细胞定位	细胞核
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthesised phosphopeptide derived from human NIPA around the phosphorylation site of Ser354:TR(p-S)WD
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The regulated oscillation of protein expression is an essential mechanism of cell cycle control. The SCF class of E3 ubiquitin ligases is involved in this process by targeting cell cycle regulatory proteins for degradation by the proteasome, with the F-box subunit of the SCF specifically recruiting a given substrate to the SCF core. NIPA (nuclear interaction partner of ALK) is a human F-box-containing protein that defines an SCF-type E3 ligase (SCFNIPA) controlling mitotic entry. Assembly of this SCF complex is regulated by cell-cycle-dependent phosphorylation of NIPA, which restricts substrate ubiquitination activity to interphase. Nuclear cyclin B1 is a substrate of SCFNIPA. Inactivation of NIPA by RNAi results in nuclear accumulation of cyclin B1 in interphase, activation of cyclin B1-Cdk1 kinase activity, and premature mitotic entry. Thus, SCFNIPA-based ubiquitination may regulate S-phase completion and mitotic entry in the mammalian cell cycle. Function: Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.  Subunit: Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-354. Interacts with CCNB1. Subcellular Location: Nuclear. Tissue Specificity: Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.  Post-translational modifications: hosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.  Similarity: Contains 1 C3HC-type zinc finger.  SWISS: Q86WB0 Gene ID: 51530 Database links: Entrez Gene: 51530 Human Entrez Gene: 232679 Mouse Entrez Gene: 296957 Rat SwissProt: Q86WB0 Human SwissProt: Q80YV2 Mouse Unigene: 194157 Human Unigene: 29780 Mouse Unigene: 474686 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Hela Cell(Human)Lysate at 30 ug Primary: Anti- p-NIPA(Ser354) (bs-8364R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55kD