产品详情
文献和实验
库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma(heparin).
规格 ：96T
产品概况
| 货号 | EK0458 |
|---|---|
| 产品名称 | Human MMP-1 ELISA Kit |
| 产品描述 | For quantitative detection of human MMP-1 in cell culture supernates, serum and plasma(heparin). |
| 免疫原 | NSO, F20-N469 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | MMP1 |
| 基因全名 | matrix metallopeptidase 1 (interstitial collagenase) |
| Uniprot ID | P03956 |
| 指标别名 | 27 kDa interstitial collagenase|CLG|CLGN|Fibroblast collagenase|Interstitial collagenase|Matrix metallopeptidase 1 (interstitial collagenase)|Matrix Metalloproteinase 1|Matrix metalloproteinase-1|MMP 1|MMP-1|MMP1|MMP1_HUMAN |
| 背景资料 | Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0458-CAP | 96-well plate precoated with anti- human MMP-1 antibody | 1 |
| EK0458-ST | Lyophilized recombinant human MMP-1 standard | 10ng/tubex2 |
| EK0458-DA | Biotinylated anti- human MMP-1 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 215 | 1557 | 4510 | 235 | 1708 | 4065 |
| Standard deviation | 13.97 | 118.33 | 261.58 | 19.5 | 160.55 | 308.94 |
| CV(%) | 6.5% | 7.6% | 5.8% | 8.3% | 9.4% | 7.6% |
Human MMP-1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.019 | 0.061 | 0.106 | 0.196 | 0.387 | 0.774 | 1.686 | 2.665 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
