CD44v3 antibody

供应商 ：上海康朗生物科技有限公司

库存 ：大量

目录编号 ：kl-4497R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：12个月

抗体英文名 ：CD44v3 antibody

抗体名 ：CD44v3蛋白抗体

宿主 ：Rabbit

适应物种 ：Human

免疫原 ：KLH conjugated synthetic peptide derived from human MUTCH 1:161-260/493

亚型 ：IgG

形态 ：冻干粉或液体

应用范围 ：ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500

浓度 ：1mg/ml

保存条件 ：-20 °C

规格 ：100ul 200ul

CD44v3 antibody

中文名称	CD44v3蛋白抗体
别    名	CD44 antigen; CD44 molecule; CD44v3; Heparan sulfate proteoglycan; IN; MUTCH 1; Phagocytic glycoprotein I.
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	细胞生物  细胞类型标志物  细胞膜蛋白
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,
产品应用	ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	51kDa
细胞定位	细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human MUTCH 1:161-260/493
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008] Function: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. There are 17 different isoforms. Subcellular Location: Membrane; single-pass type I membrane protein SWISS: P16070 Gene ID: 960 Database links: Entrez Gene: 960 Human Omim: 107269 Human SwissProt: P16070 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44v3) Polyclonal Antibody, Unconjugated (bs-4497R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).  Primary Antibody (green line): Rabbit Anti-CD44v3 antibody (bs-4497R),Dilution: 0.2 μg /10^6 cells;  Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.