NSE神经元特异性烯醇化酶/γ 烯醇化酶抗体

NSE神经元特异性烯醇化酶/γ 烯醇化酶抗体

价格: ¥1380 - 2200

品牌:LMAI Bio

货号:LM-10829R

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供应商 :上海联迈生物工程有限公司

库存 :大量

目录编号 :LM-10829R

克隆性 :多克隆

抗原来源 :Rabbit

保质期 :1年

抗体英文名 :NSE

抗体名 :神经元特异性烯醇化酶/γ 烯醇化酶抗体

宿主 :Rabbit

适应物种 :Human

免疫原 :Recombinant human NSE protein: 

亚型 :IgG

形态 :Lyophilized or Liquid

应用范围 :WB=1:500-2000 (石蜡切片需做抗原修复)

浓度 :1mg/ml

保存条件 :Store at -20 °C

规格 :100ul  200ul

NSE神经元特异性烯醇化酶/γ 烯醇化酶抗体
英文名称 NSE
中文名称 神经元特异性烯醇化酶/γ 烯醇化酶抗体
别    名 Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, 
产品应用 WB=1:500-2000 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human NSE protein: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].

Function:
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Subunit:
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular Location:
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Tissue Specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Similarity:
Belongs to the enolase family.

SWISS:
P09104

Gene ID:
2026

Database links:

Entrez Gene: 2026 Human

Entrez Gene: 13807 Mouse

Entrez Gene: 24334 Rat

Omim: 131360 Human

SwissProt: P09104 Human

SwissProt: P17183 Mouse

SwissProt: P07323 Rat

Unigene: 511915 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

神经元特异性烯醇化酶(NSE)存在于神经元细胞和神经内分泌组织中,起源于神经内分泌细胞的肿瘤可产生过量的NSE。NSE也是小细胞肺癌的检测指标,70%左右的小细胞肺癌患者血中NSE升高,而其他组织型肺癌NSE升高的患者仅为10%~20%。
产品图片
Sample: 
U-251 MG (Human) Lysate at 30 ug
Primary: Anti- NSE (bs-10829R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD


Sample: 
U251(Human) cellLysate at 40 ug
U-87MG(Human) cell Lysate at 40 ug
Primary: Anti-NSE(bs-10829R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD


Sample: 
U-87 MG (Human) Lysate at 30 ug
Primary: Anti- NSE (bs-10829R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD


Sample: 
Hep G2 (Human) Lysate at 30 ug
Primary: Anti- NSE (bs-10829R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

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