SYVN1滑膜细胞凋亡抑制物1抗体

供应商 ：上海联迈生物工程有限公司

库存 ：大量

目录编号 ：LM-0679R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：1年

抗体英文名 ：SYVN1

抗体名 ：滑膜细胞凋亡抑制物1抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat, Dog, Cow, 

免疫原 ：KLH conjugated synthetic peptide derived from human SYVN1:531-617/617 

亚型 ：IgG

形态 ：Lyophilized or Liquid

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

浓度 ：1mg/ml

保存条件 ：Store at -20 °C

规格 ：100ul  200ul

SYVN1滑膜细胞凋亡抑制物1抗体

英文名称	SYVN1
中文名称	滑膜细胞凋亡抑制物1抗体
别    名	1200010C09Rik; HRD1; KIAA1810; MGC40372; Synovial apoptosis inhibitor 1 synoviolin; Synoviolin 1 isoform b; SYVN1_HUMAN; DER3; E3 ubiquitin-protein ligase synoviolin; HMG coA reductase degradation 1 homolog; OTTHUMP00000230429; OTTHUMP00000230430; OTTHUMP00000230431; OTTHUMP00000230432; Synovial apoptosis inhibitor 1; Synoviolin 1 isoform b; SYNOVIOLIN; SYVN1.
	Specific References  (2)     |     bs-0679R has been referenced in 2 publications. [IF=4.65] Zemoura, Khaled, et al. "Endoplasmic Reticulum Associated Degradation (ERAD) Controls Cell Surface Expression of GABAB Receptors." Journal of Biological Chemistry (2013): jbc-M113.  Human.   PubMed:24114844 [IF=1.89] Yan, Shu, et al. "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." Experimental diabetes research 2012 (2011).  Rat.   PubMed:21904541
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	细胞生物  免疫学  细胞凋亡
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Cow,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	65kDa
细胞定位	细胞浆 细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human SYVN1:531-617/617
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011] Function: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Subunit: Homodimer. Interacts with p53/TP53 and HTT. Interacts with VCP, HERPUD1 and DERL1. Part of a complex containing SYVN1, HERPUD1, SELS and DERL1; which probably transfer misfolded proteins from the ER to VCP. Part of a complex containing SYVN1, SEL1L and DERL2. Interacts with UBXN6. Interacts with SEL1L; recruits ERLEC1 and OS9. May form a complex with ERLEC1; HSPA5; OS9 AND SEL1L. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). Post-translational modifications: Not N-glycosylated. Auto-ubiquitinated.  Similarity: Belongs to the HRD1 family. Contains 1 RING-type zinc finger. SWISS: Q86TM6 Gene ID: 84447 Database links: Entrez Gene: 84447 Human Entrez Gene: 74126 Mouse Entrez Gene: 361712 Rat Omim: 608046 Human SwissProt: Q86TM6 Human SwissProt: Q9DBY1 Mouse Unigene: 75859 Human Unigene: 149870 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.  与关节滑膜的损伤有关。
产品图片	Sample:  Kidney (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-SYVN1 (bs-0679R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65/70 kD Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(bs-0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(bs-0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining The figure annotation:  The blue histogram is unstained cells. The Wathet Blue histogram is cells stained with secondary antibody (bs-0295G-FITC) alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody(bs-0295P)plus secondary antibody.  The green histogram is cells stained with Rabbit Anti-SYVN1 antibody (bs-0679R) plus secondary antibody. Positive control: Hepg2 cells  Concentration: 1:50