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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma( heparin, EDTA, citrate).
样本 ：酶联免疫双抗夹心法
规格 ：96T
产品概况
| 货号 | EK0411 |
|---|---|
| 产品名称 | Mouse IL-6 ELISA Kit |
| 产品描述 | For quantitative detection of mouse IL-6 in cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| 免疫原 | E.coli, F25-T211 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | IL6 |
| 基因全名 | interleukin 6 |
| Uniprot ID | P08505 |
| 指标别名 | B cell differentiation factor|B cell stimulatory factor 2|B-cell stimulatory factor 2|BSF 2|BSF-2|BSF2|CDF|CTL differentiation factor|Cytotoxic T cell differentiation factor|Hepatocyte stimulating fact|HGF|HPGF|HSF|Hybridoma growth factor|Hybridoma plasmacytoma growth factor|IFN-beta-2|IFNB2|IL 6|IL-6|IL6|IL6 protein|IL6_HUMAN|Interferon beta 2|Interferon beta-2|Interleukin 6|Interleukin 6 (interferon beta 2)|Interleukin BSF 2|Interleukin-6|Interleukin6 |
| 背景资料 | The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0411-CAP | 96-well plate precoated with anti- mouse IL-6 antibody | 1 |
| EK0411-ST | Lyophilized recombinant mouse IL-6 standard | 10ng/tubex2 |
| EK0411-DA | Biotinylated anti- mouse IL-6 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 159 | 495 | 36 | 147 | 482 |
| Standard deviation | 2.41 | 7.79 | 35.64 | 2.91 | 7.93 | 40.48 |
| CV(%) | 6.7% | 4.9% | 7.2% | 8.1% | 5.4% | 8.4% |
Mouse IL-6 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.005 | 0.055 | 0.099 | 0.200 | 0.374 | 0.761 | 1.317 | 2.144 |
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入驻年限：17年
