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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma( heparin, EDTA, citrate).
规格 ：96T
产品概况
| 货号 | EK0397 |
|---|---|
| 产品名称 | Human IL-2 ELISA Kit |
| 产品描述 | For quantitative detection of human IL-2 in cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| 免疫原 | E.coli,A21-T153 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | IL2 |
| 基因全名 | interleukin 2 |
| Uniprot ID | P60568 |
| 指标别名 | Aldesleukin|IL 2|IL-2|IL2|IL2_HUMAN|Interleukin 2|Interleukin-2|Interleukin2|Involved in regulation of T cell clonal expansion|Lymphokine|T cell growth factor|T-cell growth factor|TCGF |
| 背景资料 | Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0397-CAP | 96-well plate precoated with anti- human IL-2 antibody | 1 |
| EK0397-ST | Lyophilized recombinant human IL-2 standard | 10ng/tubex2 |
| EK0397-DA | Biotinylated anti- human IL-2 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 158 | 484 | 28 | 169 | 474 |
| Standard deviation | 1.27 | 8.84 | 30 | 1.14 | 10.98 | 38.39 |
| CV(%) | 4.1% | 5.6% | 6.2% | 4.1% | 6.5% | 8.1% |
Human IL-2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.005 | 0.049 | 0.106 | 0.199 | 0.371 | 0.734 | 1.471 | 2.213 |
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