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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0394 |
|---|---|
| 产品名称 | Mouse IL-1 beta ELISA Kit |
| 产品描述 | For quantitative detection of mouse IL-1 beta in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli,V118-S269 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 12.5pg/ml-800pg/ml |
| 基因名称 | IL1B |
| 基因全名 | interleukin 1, beta |
| Uniprot ID | P10749 |
| 指标别名 | Catabolin|Hematopoietin 1|IFN beta inducing factor|IL 1|IL 1 beta|IL 1B|IL-1 beta|IL1|IL1 BETA|IL1B|IL1B_HUMAN|IL1F2|Interleukin 1 beta|Interleukin 1 beta precursor|Interleukin-1 beta|LAF|OAF|Osteoclast activating factor|Preinterleukin beta|Pro interleukin 1 beta |
| 背景资料 | Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0394-CAP | 96-well plate precoated with anti- mouse IL-1 beta antibody | 1 |
| EK0394-ST | Lyophilized recombinant mouse IL-1 beta standard | 10ng/tubex2 |
| EK0394-DA | Biotinylated anti- mouse IL-1 beta antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 33 | 211 | 483 | 36 | 217 | 481 |
| Standard deviation | 1.74 | 10.33 | 34.29 | 2.19 | 14.75 | 35.59 |
| CV(%) | 5.3% | 4.9% | 7.1% | 6.1% | 6.8% | 7.4% |
Mouse IL-1 beta ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 12.5 | 25 | 50 | 100 | 200 | 400 | 800 |
| O.D. | 0.063 | 0.107 | 0.143 | 0.214 | 0.390 | 0.702 | 1.285 | 2.092 |
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入驻年限：17年
