产品详情
文献和实验
相关推荐
库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Rat
规格 ：96T
产品概况
| 货号 | EK0393 |
|---|---|
| 产品名称 | Rat IL-1 beta ELISA Kit |
| 产品描述 | For quantitative detection of rat IL-1 beta in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli,V117-S268 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | IL1B |
| 基因全名 | interleukin 1, beta |
| Uniprot ID | Q63264 |
| 指标别名 | Catabolin|Hematopoietin 1|IFN beta inducing factor|IL 1|IL 1 beta|IL 1B|IL-1 beta|IL1|IL1 BETA|IL1B|IL1B_HUMAN|IL1F2|Interleukin 1 beta|Interleukin 1 beta precursor|Interleukin-1 beta|LAF|OAF|Osteoclast activating factor|Preinterleukin beta|Pro interleukin 1 beta |
| 背景资料 | The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE). |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0393-CAP | 96-well plate precoated with anti- rat IL-1 beta antibody | 1 |
| EK0393-ST | Lyophilized recombinant rat IL-1 beta standard | 10ng/tubex2 |
| EK0393-DA | Biotinylated anti- rat IL-1 beta antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 58 | 233 | 962 | 62 | 241 | 881 |
| Standard deviation | 3.65 | 11.41 | 69.26 | 4.21 | 12.29 | 68.71 |
| CV(%) | 6.3% | 4.9% | 7.2% | 6.8% | 5.1% | 7.8% |
Rat IL-1 beta ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.022 | 0.072 | 0.136 | 0.213 | 0.364 | 0.779 | 1.626 | 2.440 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
