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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum, plasma(heparin, EDTA)and urine.
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0326 |
|---|---|
| 产品名称 | Mouse EGF ELISA Kit |
| 产品描述 | For quantitative detection of mouse EGF in cell culture supernates, serum, plasma(heparin, EDTA)and urine. |
| 免疫原 | E.coli, N977-R1029 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 7.8pg/ml-500pg/ml |
| 基因名称 | EGF |
| 基因全名 | epidermal growth factor |
| Uniprot ID | P01132 |
| 指标别名 | AI790464|Beta urogastrone|Epidermal growth factor|HOMG4|OTTHUMP00000219721|OTTHUMP00000219722|Pro epidermal growth factor|Pro epidermal growth factor precursor (EGF)|URG|Urogastrone |
| 背景资料 | Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0326-CAP | 96-well plate precoated with anti- mouse EGF antibody | 1 |
| EK0326-ST | Lyophilized recombinant mouse EGF standard | 10ng/tube×2 |
| EK0326-DA | Biotinylated anti- mouse EGF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 19 | 63 | 276 | 19 | 64 | 270 |
| Standard deviation | 1.42 | 4.34 | 17.38 | 1.53 | 5.44 | 18.63 |
| CV(%) | 7.5% | 6.9% | 6.3% | 8.1% | 8.5% | 6.9% |
Mouse EGF ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.035 | 0.189 | 0.284 | 0.462 | 0.752 | 1.123 | 1.629 | 2.545 |
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