产品详情
文献和实验
相关推荐
库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).
规格 ：96T
产品概况
| 货号 | EK0307 |
|---|---|
| 产品名称 | Human BDNF ELISA Kit |
| 产品描述 | For quantitative detection of human BDNF in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, H129-R247 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | ＜4pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | BDNF |
| 基因全名 | brain-derived neurotrophic factor |
| Uniprot ID | P23560 |
| 指标别名 | Abrineurin|ANON2|Brain derived neurotrophic factor|BULN2|MGC34632|Neurotrophin |
| 背景资料 | Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0307-CAP | 96-well plate precoated with anti- human BDNF antibody | 1 |
| EK0307-ST | Lyophilized recombinant human BDNF standard | 10ng/tube×2 |
| EK0307-DA | Biotinylated anti- human BDNF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 47 | 224 | 1022 | 44 | 244 | 926 |
| Standard deviation | 3.57 | 9.63 | 44.96 | 3.78 | 12.44 | 50 |
| CV(%) | 7.6% | 4.3% | 4.4% | 8.6% | 5.1% | 5.4% |
Human BDNF ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.034 | 0.058 | 0.088 | 0.124 | 0.224 | 0.725 | 1.442 | 2.601 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
