NF-H antibody

供应商 ：上海康朗生物科技有限公司

库存 ：大量

目录编号 ：kl-0708R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：12个月

抗体英文名 ：NF-H antibody

抗体名 ：高分子量神经丝蛋白抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat,

免疫原 ：KLH conjugated synthetic peptide derived from human NF-H:1-100/1026

亚型 ：IgG

形态 ：冻干粉或液体

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test IF=1:100-500

浓度 ：1mg/ml

保存条件 ：-20 °C

规格 ：50ul 100ul 200ul

NF-H antibody

中文名称	高分子量神经丝蛋白抗体
别    名	Neurofilament 200; 200 kDa neurofilament protein; 200 kD Neurofilament Heavy; NEFH; NEFH; NF200; NF-200; Neurofilament H; Neurofilament heavy polypeptide 200kD; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; Neurofilament triplet H protein; Hypophosphorylated Neurofilament H; NF H; NFH; NFH_HUMAN; KIAA0845.
	Specific References  (3)     |     bs-0708R has been referenced in 3 publications. [IF=2.41] Cheng, Lei, et al. "Lentiviral-mediated transfer of CDNF promotes nerve regeneration and functional recovery after sciatic nerve injury in adult rats."Biochemical and Biophysical Research Communications?(2013).  IHC-P ;  Rat.   PubMed:not posted yet [IF=2.20] Wang, Nan, et al. "Myocardin-related transcription factor-A is key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells." Gene (2013).  WB ;  Rat.   PubMed:23541806 [IF=5.14] Zhao, Hua, et al. "Transplantation of Cerebral Dopamine Neurotrophic Factor Transducted BMSCs in Contusion Spinal Cord Injury of Rats: Promotion of Nerve Regeneration by Alleviating Neuroinflammation." Molecular neurobiology (2014): 1-13.  IHC-P ;  Rat.   PubMed:25421210
规格价格	50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	50ul  100ul  200ul
研究领域	细胞生物  神经生物学  信号转导  细胞凋亡  转录调节因子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test  IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	118kDa
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human NF-H:1-100/1026
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Post-translational modifications: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.  Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.  Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. DISEASE: Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Similarity: Belongs to the intermediate filament family. SWISS: P12036 Gene ID: 4744 Database links: Entrez Gene: 4744 Human Entrez Gene: 380684 Mouse Entrez Gene: 24587 Rat Omim: 162230 Human SwissProt: P12036 Human SwissProt: P19246 Mouse SwissProt: P16884 Rat Unigene: 198760 Human Unigene: 298283 Mouse Unigene: 108194 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample:  Brain (Mouse) Lysate at 30 ug Mcf-7 Cell Lysate at 30 ug Primary: Anti- NF-H (bs-0708R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD