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免疫原 :Full length plasma protein
亚型 :IgG
形态 :冻干粉
保存条件 :Store at -20℃ for one year.
克隆性 :Polyclonal
标记物 :无标记物
适应物种 :Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig
宿主 :小鼠,兔,羊,驴
应用范围 :WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
浓度 :1mg/ml
靶点 :详见说明
抗体英文名 :Rabbit anti-Rat IgM whole serum
抗体名 :兔抗大鼠IgM抗血清
规格 :50ul-100ul
上海圻明生物精选各种常用抗体,稳定可靠,性价比高。包括标签抗体,内参抗体以及兔抗大鼠IgM抗血清二抗质量可靠、高特异性的抗体已成为生物学研究中最有效和最重要的工具之一,如胞内和胞外蛋白的鉴定、定量及定位研究、流式细胞实验、免疫沉淀和免疫共沉淀、Western blot、免疫组化、免疫荧光等。此外,抗体已被直接应用于疾病诊断和治疗的研究中。产品描述Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications.br> Protein A is conjugated to 10 nm colloidal gold. This gold conjugated protein A generates electron dense particles that are visible using electron microscopy.
兔抗大鼠IgM抗血清二抗广义上是指专门和一抗进行特异性反应和结合的抗体,在免疫学实验中,经常需要针对实验需求选择不同的二抗。通常情况下,某一特定的实验中可能同时有几种二抗可供选择,如何能选择到最适合该实验的二抗,需要综合考虑以下几个方面:
1、一抗的物种来源:一抗是什么物种来源的,相应的二抗也要是抗该物种的抗体。例如,如果一抗是在小鼠体内制备的,那么就应当选择抗小鼠的二抗,如果一抗是在兔体内制备的,那么二抗就应当是抗兔的抗体。至于二抗本身是在什么动物中制备的对二抗的质量并无明显的影响。
2、一抗的类别亚型:二抗需与一抗的类别或亚型相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白,因此相应的二抗就是抗IgG抗体。单克隆抗体的类别及亚型通常会在产品列表中列出,如果你的一抗是小鼠IgM,那么相应的二抗就应当是抗小鼠IgM,或是抗小鼠IgG抗体。如果单克隆一抗是小鼠IgG的某一亚型(IgG1,IgG2a,IgG2b,IgG3),那么几乎所有的抗小鼠IgG都可以与之结合,或者你也可以选择专门针对这一亚型的二抗,例如,如果你的一抗是小鼠IgG1,那么你可以选择抗IgG1的二抗,此种抗体在双标记实验中尤其适合。如果不知道一抗是哪一类别或亚型,那么抗小鼠IgG是一个不错的选择,因为此种抗体可以识别大多数类型的IgG免疫球蛋白。
3、二抗的偶联标记:一般来讲,偶联到二抗上的探针主要有HRP、荧光素、生物素等。选用哪种探针的二抗主要取决于具体的实验。ELISA、Western Blot和免疫组化中,最常用的二抗是酶标二抗(如HRP);而免疫荧光和流式细胞术中通常使用荧光素标记的二抗,如FITC、CY3、PE等荧光素。
4、抗体的纯化方法:亲和层析纯化的抗体一般更受人们欢迎,因为这些产品的特异性较高,产生的非特异性条带和背景较低。但有些情况下也要考虑到纯化过程中IgG的损失,因为含有亲和力很高的抗体,尤其是当目的抗原存在的量很小的时候,高亲和性的IgG部分就显得尤为重要。
兔抗大鼠IgM抗血清更多相关二抗目录
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QM0297D 驴抗人IgG Donkey Anti-human IgG
QM0295D 驴抗兔IgG Donkey Anti-rabbit IgG
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QM0296R 兔抗小鼠IgG Rabbit Anti-mouse IgG
QM0295M 小鼠抗兔IgG Mouse Anti-rabbit IgG (H + L)
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QM0345G 羊抗人IgM Goat Anti-human IgM
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QM0297G 羊抗人IgG Goat Anti-human IgG
QM0326M 小鼠抗牛IgG Mouse Anti-Bov IgG
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QM0294R 兔抗羊IgG Rabbit Anti-Goat IgG
QM0336R 兔抗猴IgM Rabbit Anti-Monkey IgM
QM0297R 兔抗人IgG Rabbit Anti-human IgG
QM0309R 兔抗猪IgG Rabbit Anti-pig IgG
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QM0326R 兔抗牛IgG Rabbit Anti-Bov IgG
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QM0368Rs 兔抗小鼠IgM抗血清 Rabbit anti-mouse IgM whole serum
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QM0358Rs 兔抗豚鼠IgG抗血清 Rabbit anti-Guinea pig IgG whole serum
QM0308Rs 兔抗马IgG抗血清 Rabbit anti-horse IgG whole serum
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QM0345R 兔抗人IgM Rabbit Anti-human IgM
QM0358G 羊抗豚鼠IgG Goat Anti-Guinea pig IgG
QM0358M 小鼠抗豚鼠IgG Mouse Anti-Guinea pig IgG
QM0358R 兔抗豚鼠IgG Rabbit Anti-Guinea pig IgG
QM0358D 驴抗豚鼠IgG Donkey Anti-Guinea pig IgG
QM0368G 羊抗小鼠IgM Goat Anti-Mouse IgM
QM0368R 兔抗小鼠IgM Rabbit Anti-mouse IgM
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QM0369M 小鼠抗兔IgM Mouse Anti-Rabbit IgM
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QM0375R 兔抗豚鼠IgM Rabbit Anti-Guinea pig IgM
QM0377R 兔抗小鼠IgG-Fc Rabbit Anti-mouse IgG-Fc
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QM0295Gs 羊抗兔IgG抗血清 Goat anti-Rabbit IgG whole serum
QM0295Ds 驴抗兔IgG抗血清 Donkey anti-Rabbit IgG whole serum
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QM0358Ds 驴抗豚鼠IgG抗血清 Donkey anti-Gpig IgG whole serum
QM0310Gs 羊抗鸡IgG抗血清 Goat anti-Chicken IgG whole serum
QM0326Ms 小鼠抗牛IgG腹水 Mouse anti-Bovine IgG ascites
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QM0368Gs 羊抗小鼠IgM抗血清 Goat anti-Mouse IgM whole serum
QM0326Gs 羊抗牛IgG抗血清 Goat anti-Bovine IgG whole serum
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QM0295G-SA 链霉亲和素标记山羊抗兔IgG Goat Anti-rabbit IgG/Streptavidin
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QM0432R 兔抗鸡IgY抗体 Rabbit Anti-Chicken IgY
上海圻明生物科技有限公司
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