总RNA提取试剂盒价格_品牌:wksublo-丁香通官网

样本： 液体

标记物：总RNA

适应物种：不限

应用： 科研单位

检测方法： 酶联免疫法

检测限：不限

供应商：上海瓦兰生物

库存：大量

规格：50 次

总RNA提取试剂盒

试剂盒组成

名称	96孔配置	48孔配置	备注
微孔酶标板	12孔×8条	12孔×4条	无
标准品	0.3mL	0.3mL	无
样本稀释液	6mL	3mL	无
检测抗体-HRP	10mL	5mL	无
20×洗涤缓冲液	25mL	15mL	按说明书进行稀释
底物A	6mL	3mL	无
底物B	6mL	3mL	无
终止液	6mL	3mL	无
封板膜	2张	2张	无
说明书	1份	1份	无
自封袋	1个	1个	无

注：标准品浓度依次为：8、4、2、1、0.5、0 ng/ml.
试剂的准备
20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。
洗板方法

手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。
自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。

操作步骤

从室温平衡60min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。
设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；
待测样本孔先加待测样本10μL，再加样本稀释液40μL；
随后标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。
弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。
每孔加入底物A、B各50μL，37℃避光孵育15min。
每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。

结果判断
绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。

试剂盒性能

准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。
灵敏度：最低检测浓度小于0.1 ng/ml。
特异性：不与其它可溶性结构类似物交叉反应。
重复性：板内变异系数小于10%、板间变异系数小于15%。
贮藏：2-8℃，避光防潮保存。
有效期：6个月

免责声明

试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。
严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。

针对核酸分离纯化的卡罗登试剂盒，包括质粒DNA提取；胶DNA回收；PCR产物回收；组织、细胞、血液、植物、土壤、微生物和粪便等7种基因组DNA提取试剂盒；Karrol（RNA提取试剂）；组织、血液、植物等3种总RNA提取试剂盒。它们采用了独特的疏水膜技术和缓冲系统，使试剂盒吸附核酸的专一性和高效性都远远高于传统的硅胶膜，其性价比更是远高于其他同类试剂盒。

Karroten试剂盒具有以下特点：

◆材料需求量少：只需要少量的材料即可达到相同的效果

◆产物质量高：提取产物的完整性好，纯度高，可以直接用于下游实验

◆过程安全环保：提取过程不需要苯酚、氯仿等有毒溶剂，保护使用者的操作安全

◆操作简便快捷：提取步骤少，所需时间短

◆性价比高：单位样品的提取成本较低，远低于其他品牌
本公司是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司一方面注意跟踪世界范围分子生物学、细胞生物学等生物学科的发展前沿，努力将欧美专业生产厂家的具有水平的产品推荐给各类研究人员；另一方面也非常重视自主产品研究和开发，推出了一系列具有竞争力的产品和服务。公司客户遍布大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位。公司在重视产品质量的同时，也建立了一套集、物流、售后服务等多个部门的全方位的服务体系，努力把我们方便、快捷、周到的服务提供给每一个客户。公司主营业务：试剂盒：免疫组化试剂盒、ELISA试剂盒、分子生物学试剂盒。细胞：原装进口细胞、国产肿瘤细胞、国产正常细胞。耗材：细胞培养耗材、普通实验耗材。生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂。抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。公司代理品牌：RB .RND. Amresco 、prospec 、lifespan 、ABR、s 、spring、 abcam 、Santa Cruz、usbio BD耗材、Gibco试剂、 corning耗材 sigma试剂
其中Sigma公司是领先的生命科学与高科技公司，其生物化学与有机化学产品、试剂盒和服务被广泛应用于染色体组和蛋白质组研究等生命科学研究、生物技术、药品开发和疾病诊断以在2015年年底，Sigma-Aldrich与默克密理博合为一体。合并后的公司在美国和加拿大以MilliporeSigma名称运营，是默克集团的生命科学业务部 – 默克集团是1250亿美元生命科学行业的领头羊。

Sample collection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples should be centrifugated adequately and no hemolysis or granule was allowed.
Materials required but not supplied
1. Standard microplate reader(450nm)
2. Precision pipettes and Disposable pipette tips.
3. 37 ℃ incubator
Precautions
1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
3. Mix all reagents before using.
Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
Materials supplied

Name	96 determinations	48 determinations
Microelisa stripplate	12*8strips	12*4strips
Standard	0.3ml	0.3ml
Sample diluent	6.0ml	3.0ml
HRP-Conjugate reagent	10.0ml	5.0ml
20X Wash solution	25ml	15ml
Chromogen Solution A	6.0ml	3.0ml
Chromogen Solution B	6.0ml	3.0ml
Stop Solution	6.0ml	3.0ml
Closure plate membrane	2	2
User manual	1	1
Sealed bags	1	1

Note: Standard concentration was followed by:
480、240、120、60、30、0 μg/mL.
Reagent preparation
20×wash solution:Dilute with Distilled or deionized water 1:20.
Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not

appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results

This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
The sensitivity by this assay is 1.0 μg/mL.
Standard curve

Storage： 2-8℃.
validity： six months.

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!