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英文名 :Ribonuclease H2A E.Coli Recombinant
保质期 :1年
供应商 :上海沪震实业有限公司
保存条件 :-20°C
规格 :5μg/20μg/1mg
RNASEH2A E.ColiATALOGUE NUMBER
ENZ-713
SYNONYMS
AGS4, JUNB, RNASEHI, RNHIA, RNHL , Ribonuclease H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H(35), Ribonuclease HI large subunit, RNase HI large subunit, EC=3.1.26.4.INTRODUCTION
Ribonuclease H2 subunit A (RNASEH2A) is a member of the RNase HII family and eukaryotic subfamily. RNASEH2A Plays a part in DNA replication, probably by mediating the removal of lagging-strand Okazaki fragment RNA primers throughout DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5’-phosphomonoester and is capable to bind magnesium or manganese as cofactors. Aicardi-Goutieres syndrome type 4 (AGS4) caused by defects in RNASEH2A.DESCRIPTION
RNASEH2A Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-299) and having a molecular mass of 35.8kDa.RNASEH2A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SOURCE
Escherichia Coli.PHYSICAL APPEARANCE
Sterile filtered colorless solution.FORMULATION
The RNASEH2A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.PURITY
Greater than 85% as determined by SDS-PAGE.AMINO ACID SEQUENCE
MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL.USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.上海沪震实业有限公司
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