胶质纤维酸性蛋白抗体

克隆性 ：多克隆

抗原来源 ：Rabbit

供应商 ：上海雅吉生物科技有限公司

宿主 ：Rabbit

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:200-1000 IHC-F=1:200-1000 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复）

浓度 ：1mg/ml

规格 ：50ul/100ul/200ul

胶质纤维酸性蛋白抗体
分 子 量    48kDa
细胞定位    细胞浆 
性    状    Lyophilized or Liquid
浓    度    1mg/ml
免 疫 原    KLH conjugated synthetic peptide derived from human GFAP:51-150432 
亚    型    IgG
纯化方法    affinity purified by Protein A
储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品图片：


Sample: 
Cerebellum (Rat) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Eye (Mouse) Lysate at 40 ug
Primary: Anti-GFAP (bs-0199R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD


Sample:Optic nerve (Rat)cell Lysate at 40 ug
Primary: Anti-GFAP(bs-0199R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 53 kD

Sample: U251 Cell Lysate at 40 ug
Primary: Anti- GFAP (bs-0199R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD

胶质纤维酸性蛋白抗体产品介绍    background:This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]Function:GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial

cells.Subunit:Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).Subcellular Location:Cytoplasm. Note=Associated with intermediate filaments.Tissue Specificity:Expressed in cells lacking fibronectin.Post-translational modifications:Phosphorylated by PKN1.DISEASE:Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.Similarity:Belongs to the intermediate filament family.SWISS:P14136Gene ID:2670Database links:Entrez Gene: 281189 CowEntrez Gene: 2670 HumanEntrez Gene: 14580 MouseEntrez Gene: 24387 RatOmim: 137780 HumanSwissProt: Q28115 CowSwissProt: P14136 HumanSwissProt: P03995 MouseImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 星形胶质细胞标志物 （Astrocyte Marker）

胶质纤维酸性蛋白抗体 GFAP是一个56kDa的中间丝蛋白（intermediate filament，IF），在中枢神经系统发育期是一个特异性的标志物，以区别星形细胞和其它胶质细胞。GFAP表达在皮层和海马,急、慢性皮质酮治疗时表达减少。GFAP可以和人、大鼠、小鼠的GFAP反应，在正常和肿瘤性的星形胶质细胞阳性表达，而神经节细胞、神经元、成纤维细胞、少突胶质细胞和这些细胞来源的肿瘤细胞阴性表达，主要用于星形胶质瘤等中枢神经系统肿瘤的诊断和鉴别诊断,GFAP的缺乏可导致AD病。