免疫原 ：见官方网站

亚型 ：见官方网站

形态 ：液体或冻干粉

保存条件 ：-20°C

克隆性 ：多克隆

标记物 ：见官方网站

适应物种 ：见官方网站

保质期 ：6个月

抗原来源 ：Synthetic

目录编号 ：B-0160

级别 ：高

库存 ：大量

供应商 ：上海信裕生物科技有限公司

宿主 ：Synthetic

应用范围 ：见官方网站

浓度 ：见官方网站

靶点 ：见官方网站

抗体英文名 ：Benzamil hydrochloride

抗体名 ：Benzamil hydrochloride

规格 ：10 mg, 25 mg, 50 mg

Benzamil hydrochloride

Benzylamiloride hydrochloride
An Inhibitor of Na⁺/Ca²⁺ Exchangers and a Blocker of ASIC, TRPP3 and TRPA1 Channels
Cat #: B-0160
Sizes: 10 mg, 25 mg, 50 mg
Source: Synthetic
MW: 356.21 Da.
Target: Na⁺/Ca²⁺ exchangers, ASIC/ENaC, TRPP3, TRPA1 channels
CLICK HERE TO RECEIVE A 10 mg FREE TRIAL SAMPLE!

Benzamil is a potent amiloride analogue that acts as an inhibitor of Na⁺/Ca²⁺ exchangers and a blocker of ASIC/DEG/ENaC, TRPP3, and TRPA1 channels displaying IC₅₀ of 0.1, 1.1 and 55 µM respectively^1-3.

Benzamil is commercially available as an inhibitor for acid sensing ion channels (ASICs) belonging to Deg/ENaC family. The compound is also well known as a diuretic compound that is used for the management of congestive heart disease or hypertension. Intrathecal administration of benzamil demonstrates antinocicepitve effects in several pain models in animals and humans through the inhibition of acid sensing ion channels⁴.

Alomone Labs is pleased to offer Benzamil hydrochloride (#B-160).

References

1. Fischer, K.G. et al. (2002) Nephrol. Dial. Transplant 17, 1742.
2. Dai, X.Q. et al. (2007) Mol. Pharmacol. 72, 1576.
3. Banke, T.G. (2011) Brain Res. 1381, 21.
4. Jeong. S. et al. (2013) J. Korean Med. Sci. 28, 1238.

• Our Bioassay
• Specifications
• Related Products

Our bioassay

Alomone Labs Benzamil hydrochloride inhibits ASIC3 channels expressed in Xenopus oocytes.

A. Time course of Benzamil hydrochloride (#B-160) inhibition of ASIC3 channels current. Membrane potential was held at -60 mV, current was elicited every 50 sec by transient applications of pH 5.5, and was inhibited significantly by a 4 min application of 50 µM Benzamil hydrochloride (horizontal bar). B. Superimposed traces of ASIC3 currents upon application of control and of 50 µM Benzamil hydrochloride, as indicated, taken from the experiment in A.

Alomone Labs Benzamil hydrochloride inhibits TRPA1 currents induced by AITC in HEK-293 cells.
Dose response curve of Benzamil hydrochloride (#B-160) inhibition of human TRPA1-mediated increase in intracellular Ca²⁺. Cells were loaded with Calcium 5 dye, incubated with Benzamil hydrochloride, and stimulated with 1 µM allyl isothiocyanate (EC₈₀). Changes in intracellular Ca²⁺ following stimulation were detected as changes in relative fluorescence (RFU).
Purity>99%.
Effective concentration1-300 µM.
Chemical name3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide;hydrochloride.
Molecular formulaC₁₃H₁₅Cl₂N₇O.
CAS number161804-20-2.
ActivityBenzamil is a potent amiloride analogue, an inhibitor of the Na⁺/Ca²⁺ exchanger (IC₅₀ = 0.1 µM) ¹, a blocker of ASIC/Deg/ENaC channels ², of TRPP3 channels (IC₅₀ = 1.1 µM) ³ and of TRPA1 channels (IC₅₀ = 55 µM)⁴.
Storage before reconstitutionStore at +4^oC.
Reconstitution

Up to 100 mM in DMSO.
Centrifuge all products before use (10000 x g 5 min).

Storage after reconstitutionUp to three months at -20°C.