免疫原 ：见官方网站

亚型 ：见官方网站

形态 ：液体或冻干粉

保存条件 ：-20°C

克隆性 ：多克隆

标记物 ：见官方网站

适应物种 ：见官方网站

保质期 ：6个月

抗原来源 ：Synthetic peptide

目录编号 ：STP-200

级别 ：高

库存 ：大量

供应商 ：上海信裕生物科技有限公司

宿主 ：Synthetic peptide

应用范围 ：见官方网站

浓度 ：见官方网站

靶点 ：见官方网站

抗体英文名 ：PcTx-1

抗体名 ：PcTx-1

规格 ：50 µg, 0.1 mg, 0.5 mg, 1 mg, 5 mg

PcTx-1

Psalmotoxin-1, π-theraphotoxin-Pc1a
A Potent Blocker of ASIC1a Channels
Cat #: STP-200
Sizes: 50 µg, 0.1 mg, 0.5 mg, 1 mg, 5 mg
Source: Synthetic peptide
MW: 4695 Da.
Net Peptide Content: 100%
Target: ASIC1a channels
CLICK HERE TO RECEIVE A 50 µg FREE TRIAL SAMPLE!

Native Psalmotoxin1 (PcTx1) is a 40-amino acid toxin originally isolated from the venom of the South American tarantula Psalmopoeus cambridgei.¹

PcTX1 is characterized by the unusual quadruplet Lys²⁵-Arg²⁶-Arg²⁷-Arg²⁸, which probably forms a strongly positive “patch” at the surface of the toxin molecule, constituting an area that is a strong candidate for channel binding site recognition. The molecular scaffold of PcTX1 is likely to be similar to that previously described for both cone snail and spider toxins^5,6 and comprises a triple-stranded antiparallel β-sheet structure reticulated by three disulfide bridges.¹

PcTx1 potently (IC₅₀ = 0.9 nM) and specifically blocks a particular subclass of H⁺-gated cation channels, the Acid-sensing ion channels 1a (ASIC1a) and is able to discriminate between the two splice variant subtypes and not block the ASIC1b channel (which differs only in its N-terminal sequence). Moreover PcTX1 loses its capacity to block ASIC1a as soon as this subunit is associated with another member of the family (ASIC2a or ASIC3).¹

The ASICs are abundant in the brain and spinal cord neurons and are implicated in pain sensation, ischemic stroke mechanosensation, learning and memory.^2,3 In acute and neuropathic pain models, specific blockage of ASIC1a with PcTx1 results in analgesic effects working upstream of the opiate receptors.⁴

Using an iodinated form of the toxin, the binding for the PcTX1 site identifies at the cysteine-rich domains I and II (CRDI and CRDII) of the extracellular loop of ASIC1a. A Disulphide bridge between domains 1 and 4 of the channel supports a close relationship between the CRDI4–CRDII domain, where PcTx1 acts, and the post-M1 region, the binding site for the proton.⁷

Alomone Labs is pleased to offer PcTx-1 (#STP-200), a synthetic peptide.

References

1. Escoubas, P. et al. (2000) J. Biol. Chem. 275, 25116.
2. Sluka, S.P. et al. (2007) Pain 129, 102.
3. Jastil, J. et al. (2007) Nature 449, 316.
4. Mazzuca, M. et al. (2007) Nat. Neurosci. 10, 943.
5. Narasimhan, L. et al. (1994) Nat. Struct. Biol. 1, 850.
6. Pallaghy, P. K. et al. (1994) Protein Sci. 3, 1833.
7. Salinas, M. et al. (2005) J. Physiol. 570, 339.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Our Bioassay
• Specifications
• Citations
• Related Products

Our bioassay

Alomone Labs PcTx-1 inhibits cation currents of ASIC1a expressed in Xenopus oocytes.
Membrane potential was held at 5 mV and whole cell currents were continuously recorded using TEVC. ASIC1a currents were elicited by rapid (~2 sec) exposure to pH 5 in physiological solution (indicated by arrows). During the time indicated by the horizontal bar, 5 nM, 10 nM and 20 nM PcTx-1 (#STP-200) were introduced into the bath via perfusion. 20 nM PcTx-1 resulted in a complete, yet fairly reversible inhibition of the pH dependent inward transients.
OriginPsalmopoeus cambridgei (Trinidad chevron tarantula).
Purity>99%.
Effective concentration1-10 nM.
SequenceEDCIPKWKGCVNRHGDCCEGLECWKRRRSFEVCVPKTPKT.
ModificationsDisulfide bonds between Cys³-Cys¹⁸, Cys¹⁰-Cys²³ and Cys¹⁷-Cys³³.
Molecular formulaC₂₀₀H₃₁₂N₆₂O₅₇S₆.
CAS numberNot determined.
ActivityPsalmotoxin-1 inhibits cation currents mediated by acidsensing ion channels (ASIC1a)^1,2.
Storage before reconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
ReconstitutionAny aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitutionUp to four weeks at 4°C or three months at -20°C.
Product citations

1. Gonzalez-Inchauspe, C. et al. (2017) J. Neusosci. 37, 2589.
2. Wu, P.Y. et al. (2013) J. Neurosci. 33, 1828.
3. Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
4. Sun, X. et al. (2011) Brain Res. 1396, 77.
5. Wu, W.N. et al. (2011) Br. J. Pharmacol. 164, 1445.
6. Springauf, A. and Gründer, S. (2009) J. Physiol. 588.5, 809.