Anti-QRFP Receptor (extracellular)

QRFPR, Pyroglutamylated RFamide Peptide Receptor, Orexigenic Neuropeptide QRFP Receptor, G-Protein Coupled Receptor 103, Peptide P518 Receptor, GPR103, SP9155, AQ27
Cat #: AGR-033
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP, LCI
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-QRFP Receptor (extracellular) (#AGR-033) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the receptor in living cells. It has been designed to recognize GPR103 from rat, mouse, and human samples.

Applications
Specifications
Scientific Background

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3), mouse brain membranes (lanes 2 and 4) and human LNCaP cell lysate (lanes 3 and 6):
1-3. Anti-QRFP Receptor (extracellular) antibody (#AGR-033), (1:500).
4-6. Anti-QRFP Receptor (extracellular) antibody, preincubated with the control peptide antigen.
lmmunogen
Peptide (C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP Receptor (Accession P83858). Extracellular, N-terminus.

HomologyMouse – identical; human – 13/17 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by mass-spectrography and amino acid analysis.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

QRFP is a 26-amino acid peptide (QRFP-26) that widely distributed among vertebrates a member of the RFamide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH₂) sequence motif in their carboxyl C terminal end. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP Receptor has an extracellular N-terminal tail and intracellular C-terminus.

Its QRFP N-terminus binds with a high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis. QRFP has been shown to affect locomotor activity, energy homeostasis, reproduction, bone formation, blood pressure, hormone regulation, nociception, and appetite stimulation.

QRFP expressing neurons have shown to be expressed in various hypothalamic nuclei including the ventromedial hypothalamic nucleus, the lateral hypothalamic area, retrochiasmatic area and the arcuate nucleus^1,2. The receptor is detected in the CNS and in peripheral tissues, notably in bone, heart, kidney, pancreas and testis.

Increasing data suggest the ligand/receptor system plays an important role in the regulation of food intake and glucose homeostasis¹.

References