Anti-Doublestranded DNA

价格： $595

品牌：丹麦BioPorto Diagnostics A/S

货号：HYB 331-01

产品详情

Anti-Doublestranded DNA

Cat. No.

HYB 331-01

Product name

Anti-Doublestranded DNA

Description

Mouse monoclonal antibody

Specificity

Double stranded DNA (dsDNA). Cross-reactions to single stranded DNA (ssDNA) and very weak reactivity with RNA on dot blots has been observed.

Immunogen

F1 hybrid from NZB Black and White mouse.

Application

IB, ELISA, IF

Species reactivity (positive)

Not determined

Species reactivity (negative)

Not determined

Subclass

IgG2a/k

Clone number

35 | 9 DNA

Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

In combination with immunofluorescence the antibody is well suited for detection of dsDNA in fx. Crithidia luciliae, a monoflagellate protozoa, containing a giant mitochondrion (3).The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays (2).Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding HYB 331-01 is >16 bases and there is an inverse proportionality between binding and ionic strength (1).