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免疫原 :KLH conjugated synthetic(具体详情咨询我们)
亚型 :IgG
形态 :Lyophilized or Liquid
保存条件 :Store at -20 °C
克隆性 :多克隆
标记物 :有各种标记抗体需要可以联系我公司
适应物种 :Hu Mo Pig Cow Hor Sheep等
宿主 :Rabbit
应用范围 :WB=1:100-500,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg |test,IF=1:100-500,ICC=1:100-506,
浓度 :1mg/ml
靶点 :详询
抗体英文名 :HMGB1
抗体名 :高迁移率族蛋白B1抗体
规格 :100ul
英文名称HMGB1中文名称高迁移率族蛋白B1抗体
别 名Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
文献引用
规格50ul 100ul 200ul
研究领域肿瘤 细胞生物 免疫学 转录调节因子 结合蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量25kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HMGB1:75-170/215
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models
Function:
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Subunit:
Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.
Subcellular Location:
Nucleus. Chromosome.
Similarity:
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
SWISS:
P09429
Gene ID:
3146
Database links:
Entrez Gene: 282691 Cow
Entrez Gene: 3146 Human
Entrez Gene: 100862258 Mouse
Entrez Gene: 15289 Mouse
Entrez Gene: 25459 Rat
Omim: 163905 Human
SwissProt: P10103 Cow
SwissProt: P09429 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
近来的研究表明称之为高迁移率族蛋白B-1(HMG-B1)的核内结构蛋白在核外表达时是一种有效的早期炎症介质。
高迁移性B1组蛋白(HMGB1): 是一种核结合蛋白,在DNA重组、修复、复制和基因转录中起作用。HMGB1也是巨噬细胞分泌的一种介质。
此外,高迁移性B1组蛋白亦被受刺激的巨噬细胞或单核细胞主动分泌。在这一主动分泌过程中,HMGB1首先经乙酰化并由核内转移至溶酶体内,继而在ATP和溶血磷脂胆碱两种分泌信号指导下转移至细胞外。由坏死细胞被动释放的HMGB1和炎症细胞主动分泌的HMGB1存在分子上的差异。胞外的HMGB1可作为细胞因子参与信号传导,因为它既可识别Toll 样受体(TLR)家族的一些成员,又能与识别晚期糖基化终末产物受体(RAGE)相作用。
HMGB1能启动炎症反应,包括产生多种细胞因子、对某些干细胞产生趋化作用、诱导血管粘附分子、削弱肠上皮细胞的功能等。
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