Anti-BrdU 5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体

免疫原 ：KLH conjugated synthetic（具体详情咨询我们）

亚型 ：IgG

形态 ：Lyophilized or Liquid

保存条件 ：Store at -20 °C

克隆性 ：多克隆

标记物 ：有各种标记抗体需要可以联系我公司

适应物种 ：Hu Mo Pig Cow Hor Sheep等

宿主 ：Rabbit

应用范围 ：WB=1:100-500,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg|Test,IF=1:100-502,

浓度 ：1mg/ml

靶点 ：详询

抗体英文名 ：BrdU

抗体名 ：5-溴脱氧尿嘧啶核苷|增殖标志物单克隆抗体

规格 ：100ul

英文名称BrdU
中文名称5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体
别    名Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.  
文献引用：

规格价格50ul  100ul  
产品类型药物与化合物抗体 
研究领域细胞生物  免疫学  发育生物学  染色质和核信号  神经生物学  药物及化合物  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号1A7
交叉反应 Rat, BrdU
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量0.3071kDa
细胞定位细胞核 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原bromodeoxyuridine conjugated to KLH: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体产品介绍background:
Proliferation Marker
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). 

Subcellular Location:
Nuclear.

SWISS:
N/A

CAS:
59-14-3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体增殖标志物
BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。
用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Mouse Anti-BrdU(A7) Monoclonal Antibody, Unconjugated(bsm-0917M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: The primary antibody was Mouse Anti-BrdU(A7) Monoclonal Antibody, FITC conjugated (bsm-0917M-FITC) 1:200, 40 minutes at 37°C; The secondary antibody was Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37°C.