Anti-Complement factor B (human)

价格： $410

品牌：BioPorto

货号：HYB 008-020

产品详情

Anti-Complement factor B (human)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
官方网站：www.bioporto.com

Cat. No.

HYB 008-020

Product name

Anti-Complement factor B (human)

Description

Mouse monoclonal antibody

Specificity

HYB 008-02 is specific for the Bb fragment of human factor B. Antibody binding abolishes the formation of C3bBb complexes.

Immunogen

Complement factor B purified from human plasma

Application

ELISA, WB

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

629

Clone number

9B8

Epitope

Differs from that of HYB 008-06. Also differs from HYB 008-04 and HAV 005-02, but with slight overlap as determined by some mutual inhibition of antibody binding.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Factor B is a component of the alternative pathway of complement activation. The protein consists of a single polypeptide chain of molecular mass 93 kDa, and has a concentration in plasma of approximately 200 µg/mL. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Together with C3b, Bb forms the alternative pathway C3 convertase C3bBb.
Application 1: ELISA
HYB 008-02 reacts strongly with human factor B. A strong reaction is seen when used as a biotinylated detection antibody in sandwich ELISA with a polyclonal capture antibody against factor B (e.g. Dako A0343).
Application 2: WB
After SDS-PAGE, HYB 008-02 reacts with the Bb fragment of factor B in its non-reduced form only. HYB 008-02 can be used for the affinity purification of human factor B, when coupled to a suitable matrix.