免疫原 ：见官方网站

亚型 ：见官方网站

形态 ：冻干粉或液体

保存条件 ：-20ºC

克隆性 ：多克隆

标记物 ：见官方网站

适应物种 ：见官方网站

保质期 ：1年

抗原来源 ：见官方网站

目录编号 ：ABS 061-096

级别 ：见官方网站

库存 ：30

供应商 ：上海泽叶生物科技有限公司

宿主 ：Human, pig, rat

应用范围 ：ELISA

浓度 ：1 mg/ml

靶点 ：见官方网站

抗体英文名 ：Anti-Glucagon (human, pig, rat)

抗体名 ：Anti-Glucagon (human, pig, rat)

规格 ：200ug

Anti-Glucagon (human, pig, rat)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
官方网站：www.bioporto.com

Cat. No.

ABS 061-096

Product name

Anti-Glucagon (human, pig, rat)

Description

Mouse monoclonal antibody

Specificity

ABS 061-09 binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.

Immunogen

Synthetic C-terminal fragment of glucagon coupled to carrier

Application

ELISA

Species reactivity (positive)

Humna, pig, rat

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

2641, 24952, 397595

Clone number

09

Epitope

The epitope lies within the C-terminal region of glucagon.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2 in the L cells of the intestinal mucosa.

Application 1

ELISA
ABS 061-09 binds free glucagon in solution. 
ABS 061-09 when used as capture antibody forms a sandwich ELISA pair with ABS 058-08 as biotinylated detection antibody for measuring glucagon.
 

The calibration curve of a sandwich assay for Glucagon using ABS 061-09 as the capture antibody and ABS 058-08B as the biotinylated detection antibody.

Application 2

IHC
ABS 061-09 has been used in IHC.

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 061-09. Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000

Application 3

IF
ABS 061-09 has been used in IF.

IF staining in human pancreas tissue stained with ABS 061-09. Signal is strong in alpha cells. Dilution: 1:4000.