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免疫原 :见官方网站
亚型 :见官方网站
形态 :冻干粉或液体
保存条件 :-20ºC
克隆性 :多克隆
标记物 :见官方网站
适应物种 :见官方网站
保质期 :1年
抗原来源 :见官方网站
目录编号 :HYB 190-011
级别 :见官方网站
库存 :30
供应商 :上海泽叶生物科技有限公司
宿主 :Human, bovine, Torpedo marmorata
应用范围 :ELISA, WB, IHC
浓度 :1 mg/ml
靶点 :见官方网站
抗体英文名 :Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
抗体名 :Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
规格 :200ug
Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 190-011
- Product name
- Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 190-01 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE.
- Immunogen
-
C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.
- Application
- ELISA, WB, IHC
- Species reactivity (positive)
- Human, bovine, Torpedo marmorata
- Species reactivity (negative)
- Electric eel, human
- Subclass
-
IgG1/l
- Gene ID
- 43, 540446
- Clone number
- 4E11
- Epitope
-
C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.- Application 1
-
ELISACan be used in ELISA on amniotic fluid for the diagnosis of neural tube defects.HYB 190-01 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction) (1).
- Application 2
-
WB
In Western blotting and dot blotting HYB 190-01 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE..In Western blotting a dilution guideline of 1/75 has proved successful (1).
- Application 3
-
IHC
HYB 190-01 can be uesd in Immunohistochemistry.Human placenta stained with HYB 190-01
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入驻年限:9年