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免疫原 :见官方网站
亚型 :见官方网站
形态 :冻干粉或液体
保存条件 :-20ºC
克隆性 :多克隆
标记物 :见官方网站
适应物种 :见官方网站
保质期 :1年
抗原来源 :见官方网站
目录编号 :ABS 033-10B
级别 :见官方网站
库存 :30
供应商 :上海泽叶生物科技有限公司
宿主 :Human
应用范围 :ELISA, IHC-P
浓度 :1 mg/ml
靶点 :见官方网站
抗体英文名 :Anti-GLP-1(7-37) and GLP-1(7-36)amide (N-terminal specific)
抗体名 :Anti-GLP-1(7-37) and GLP-1(7-36)amide (N-terminal specific)
Anti-GLP-1(7-37) and GLP-1(7-36)amide (N-terminal specific)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- ABS 033-10B
- Product name
- Anti-GLP-1(7-37) and GLP-1(7-36)amide (N-terminal specific)
- Description
-
Mouse monoclonal antibody, biotinylated
- Specificity
-
ABS 033-10 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. ABS 033-10 cross-reacts with Liraglutide.
- Immunogen
-
Synthetic GLP-1(7-17)
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG2a/k
- Gene ID
- 2641
- Clone number
- 10
- Epitope
-
Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.
- Content
-
50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Biotinylated
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
- Application 1
-
ELISA
ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.This uses HYB 147-06 as capture antibody in combination with biotinylated detection antibody ABS 033-10 exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide. Specimens must be collected into tubes containing an adequate amount of DPP-4 inhibitor (in addition to routine peptidase inhibitors) to prevent the rapid in vitro degradation of the active peptide. The calibration curve of a sandwich assay for Non-amidated GLP-1 using ABS 046-03 as the capture antibody and ABS 033-10B as the biotinylated detection antibody.
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入驻年限:9年