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保存条件 ：-20℃
保质期 ：6个月
英文名 ：TOPFlash
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
TOPFlash载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | TOPFlash |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
载体质粒图谱和多克隆位点信息
载体简介
The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fatedetermination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling inDrosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. Dishevelleddown regulates glycogen synthase kinase-3 (GSK-3 ) activity. This results in the stabilization and accumulation ofcytosolic -catenin, a GSK-3 substrate, which when phosphorylated by GSK-3, is targeted for ubiquitin-mediatedproteolysis. -catenin then translocates to the nucleus, where in complexes with members of the Tcf/LEF family oftranscription regulators, activates transcription of Tcf-responsive genes. This Tcf-reporter plasmid, along withFOPFLASH (Cat. # 21-169) enables quantitation of Wnt/Wg signaling in cells transfected with these constructs.
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入驻年限：12年
