产品详情
文献和实验
相关推荐
保存条件 ：-20℃
保质期 ：6个月
英文名 ：pK18mobSacB
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pK18mobSacB载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pK18mobSacB |
| 质粒类型: | 芽孢杆菌基因敲除载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5.6kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Kanamycin |
| 筛选标记: | -- |
| 备注: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
载体质粒图谱和多克隆位点信息
载体简介
Cloning vector allowing mobilization into a wide range of GramandGram+ bacteria. Aftermobilization, the plasmid can be maintained by integration into the host chromosome viahomologous recombination. Excision of the intervening plasmid sequence by a double crossoverevent can be facilitated by selection on medium containing 10% sucrose.The sacB gene hasbeen modified to eliminate the HindIII and EcoRI sites in the coding region.Designation: pK18mobsacB
载体序列
LOCUS pK18mobsacB 5721 bp DNA 17-AUG-2006DEFINITION Cloning vector pK18mobsacB.ACCESSIONVERSIONKEYWORDS .SOURCE Cloning vector pK18mobsacB. ORGANISM Cloning vector pK18mobsacB Unclassified.REFERENCE 1 (bases 1 to 5721) AUTHORS Schafer,A., Tauch,A., Jager,W., Kalinowski,K., Thierbach,G. and Puhler,A. TITLE Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum JOURNAL Gene 145, 69-73 (1994)REFERENCE 2 (bases 1 to 5721) AUTHORS Ishikawa,J. TITLE Unofficial nucleotide sequence of pK18mobsacB JOURNAL UnpublishedREFERENCE 3 (bases 1 to 5721) AUTHORS Ishikawa,J. TITLE Direct Submission JOURNAL Submitted (17-AUG-2006) Bioactive Molecules, NIIDFEATURES Location/Qualifiers source 1..5721 /organism="Cloning vector pK18mobsacB" /mol_type="Other DNA" CDS complement(185..469) /gene="lacZ" /codon_start=1 /product="beta-galactosidase" oriT complement(2209..2736) /note="RP4 origin of transfer" CDS complement(2886..4307) /gene="sacB" /codon_start=1 /product="levansucrase" CDS complement(4799..5593) /gene="neo" /note="confers resistances to neomycin and kanamycin" /codon_start=1 /product="aminoglycoside 3'-O-phosphotransferase"BASE COUNT 1343 a 1426 c 1414 g 1538 tORIGIN 1 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc 61 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt 121 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct 181 tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctagc ttatcgcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct 481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1561 cttttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccc 1621 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag 1681 ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc 1741 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg 1801 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga 1861 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctgatag aaacagaagc 1921 cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg 1981 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc 2041 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg 2101 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat 2161 tttttgagtt atcgagattt tcaggagctc tttggcatcg tctctcgcct gtcccctcag 2221 ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa aacagcaggg 2281 aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 2341 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 2401 tggtgtatcc aacggcgtca gcggggcagg ataggtgaag taggcccacc cgcgagcggg 2461 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 2521 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc 2581 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga 2641 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc 2701 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gggcgtcccg 2761 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga 2821 tggcaggttg ggcgtcgctt ggtcggtcat ttcgctcggt accatcggca ttttcttttg 2881 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat 2941 gttttcttgc ctttgatgtt cagcaggaag ctcggcgcaa acgttgattg tttgtctgcg 3001 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt 3061 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca 3121 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc 3181 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg 3241 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact 3301 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca 3361 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt 3421 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa 3481 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg 3541 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag 3601 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt 3661 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact 3721 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa 3781 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt 3841 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc 3901 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc 3961 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca 4021 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag 4081 atatttttaa ttgtggacga atcaaattca gaaacttgat atttttcatt tttttgctgt 4141 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat 4201 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta 4261 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc 4321 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag 4381 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac 4441 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta 4501 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt 4561 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct 4621 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc 4681 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg 4741 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag cgaaccccag agtcccgctc 4801 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 4861 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 4921 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 4981 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 5041 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga 5101 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 5161 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 5221 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 5281 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 5341 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 5401 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 5461 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 5521 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 5581 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc 5641 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct 5701 taccagaggg cgccccagct g
上海钰博生物科技有限公司
品牌商实名认证
金牌会员
入驻年限：12年
