pBBR1MCS

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pBBR1MCS

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pBBR1MCS

pBBRMCS1载体基本信息

出品公司:	Ybscience
载体名称:	pBBR1MCS, pBBRMCS1, pBBRMCS, pBBR1MCS-1, pBBRMCS-1
质粒类型:	广宿主蛋白表达或克隆载体质粒，广宿主载体质粒
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	--
载体大小:	4.7 kb
5' 测序引物及序列:	M13-F: TGTAAAACGACGGCCAGT
3' 测序引物及序列:	M13-R: CAGGAAACAGCTATGAC
载体标签:	--
载体抗性:	氯霉素
筛选标记:	--
克隆菌株:	DH5α 等
宿主细胞（系）:	多种微生物物种，是广宿主质粒。
备注:	参考文献： Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2. GenBank sequence：XXU02374
稳定性/瞬表达:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

pBBRMCS1载体质粒图谱和多克隆位点信息

pBBRMCS1载体简介

 

pBBRMCS1载体序列

LOCUS       pBBR1MCS	4707 bp 	DNA  circular	SYNDEFINITION  pBBR1MCSACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     ORIGDB|GenBankCOMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..4707                     /organism="pBBR1MCS"                     /mol_type="other DNA"     CDS             complement(1697..2212)                     /label="ORF frame 3"     CDS             1781..2455                     /label="ORF frame 2"     gene            complement(2915..4234)                     /label="CAT/CamR (variant)"                     /gene="CAT/CamR (variant)"     misc_feature    complement(3033..3176)                     /label="lacZ_a"     misc_feature    3147..3169                     /label="M13_pUC_fwd_primer"     promoter        3162..3178                     /label="M13_forward20_primer"     promoter        3188..3206                     /label="T7_promoter"     misc_feature    3239..3255                     /label="pBluescriptSK_primer"     misc_feature    complement(3290..3306)                     /label="pBluescriptKS_primer"     promoter        complement(3334..3353)                     /label="T3_promoter"     promoter        complement(3370..3388)                     /label="M13_reverse_primer"     misc_feature    complement(3387..3409)                     /label="M13_pUC_rev_primer"     promoter        complement(3423..3452)                     /label="lac_promoter"     CDS             complement(3545..4234)                     /label="ORF frame 3"     CDS             complement(4487..1192)                     /label="ORF frame 3"ORIGIN    1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg   61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt  121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg  181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg  241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca  301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt  361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg  421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt  481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg  541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct  601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg  661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc  721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca  781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg  841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg  901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc  961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc 1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga 1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc 1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt 1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg 1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac 1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc 1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg 1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg 1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag 1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta 1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg 1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc 1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac 1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga 1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct 1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac 1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt 2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt 2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag 2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct 2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga 2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac 2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct 2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt 2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg 2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga 2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg 2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa 2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa 2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag 2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc 2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg 3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc 3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga 3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct 3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata 3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat 3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg 3721 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac 3781 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg 3841 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat 3901 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt 3961 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt 4021 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct 4081 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg 4141 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat 4201 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc 4261 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac 4321 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat 4381 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt 4441 cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 4501 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc 4561 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg 4621 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca 4681 gaacgggctt caggcgctcc cgaaggt