pBTB-1

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pBTB-1

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pBTB-1

载体基本信息

出品人:	Ybscience.
载体名称:	pBTB-1
质粒类型:	广宿主载体；克隆载体；原核表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	araBAD
载体大小:	3784 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Ampicillin
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       DQ058729                3784 bp    DNA     circular SYN 08-MAY-2006DEFINITION  Broad host range vector pBTB-1, complete sequence.ACCESSION   DQ058729VERSION     DQ058729.1  GI:69048308  AUTHORS   Lynch,M.D. and Gill,R.T.  TITLE     Direct Submission  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO            80309, USAFEATURES             Location/Qualifiers     source          1..3784                     /organism="Broad host range vector pBTB-1"                     /mol_type="other DNA"                     /db_xref="taxon:334961"     regulatory      60..96                     /regulatory_class="terminator"                     /note="SoxR terminator"     gene            872..1534                     /gene="rep"                     /note="rep gene from pBBR1, GenBank Accession Number                     X66730"     CDS             872..1534                     /gene="rep"                     /note="required for replication"                     /codon_start=1                     /transl_table=11                     /product="Rep"                     /protein_id="AAY99717.1"                     /db_xref="GI:69048309"                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP                     RR"     gene            1631..2491                     /gene="bla"                     /note="beta-lactam resistance"     CDS             1631..2491                     /gene="bla"                     /codon_start=1                     /transl_table=11                     /product="beta-lactamase"                     /protein_id="AAY99718.1"                     /db_xref="GI:69048310"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW"     regulatory      2507..2538                     /regulatory_class="terminator"                     /note="TonB terminator"     gene            complement(2566..3495)                     /gene="araC"     CDS             complement(2566..3495)                     /gene="araC"                     /note="regulator"                     /codon_start=1                     /transl_table=11                     /product="AraC"                     /protein_id="AAY99719.1"                     /db_xref="GI:69048311"                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"     regulatory      3503..3770                     /regulatory_class="promoter"                     /note="pBAD promoter"ORIGIN              1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa     1621 ggaagctaaa atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt     1681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca     1741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag     1801 tttacgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc     1861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca     1921 gaatgacttg gttgagtact caccagtcac agaaaagcat ctcacggatg gcatgacagt     1981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct     2041 ggcaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt     2101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga     2161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact     2221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggatc     2281 acttctgcgc tcggccctcc cggctggctg gtttattgct gataaatctg gagccggtga     2341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgcatcgt     2401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga     2461 gataggtgcc tcactgatta agcattggta atgaggatcc ccctcaagtc aaaagcctcc     2521 ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat gatttttatg acaacttgac     2581 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt     2641 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac     2701 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc     2761 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga     2821 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg     2881 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc     2941 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc     3001 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg     3061 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt     3121 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg     3181 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc     3241 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag     3301 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt     3361 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag     3421 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa     3481 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac     3541 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac     3601 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca     3661 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg     3721 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagaatt     3781 cgtc