pMHE2

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pMHE2

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pMHE2

载体基本信息

出品人:	Ybscience
载体名称:	pMHE2
质粒类型:	广宿主载体；细菌表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	T7/lac
载体大小:	5878 bp
5' 测序引物及序列:	T7 promoter 5’TAATACGACTCACTATAGGG 3’
3' 测序引物及序列:	T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
载体标签:	6XHis tag (N ter)、EK site
载体抗性:	链霉素
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       AY299693                5878 bp    DNA     circular SYN 10-FEB-2004DEFINITION  Broad host range expression vector pMHE2, complete sequence.ACCESSION   AY299693VERSION     AY299693.1  GI:34334140REFERENCE   1  (bases 1 to 5878)  TITLE     Modular broad-host-range expression vectors for single-protein and            protein complex purification  JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)   PUBMED   14766546  AUTHORS   Fodor,B.D.  TITLE     Direct Submission  JOURNAL   Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of            Sciences, Temesvari krt., Szeged, Csongrad 6701, HungaryFEATURES             Location/Qualifiers     source          1..5878                     /organism="Broad host range expression vector pMHE2"                     /mol_type="genomic DNA"                     /db_xref="taxon:244051"     primer_bind     207..225                     /note="T7 promoter primer"     regulatory      207..223                     /regulatory_class="promoter"                     /note="T7 promoter"     misc_feature    224..249                     /note="lac operator"     regulatory      280..286                     /regulatory_class="ribosome_binding_site"     misc_feature    294..296                     /note="fusion protein start codon"     misc_feature    300..317                     /note="His-tag"     misc_feature    318..329                     /note="Factor Xa cleavage site"     primer_bind     complement(428..445)                     /note="T7 terminator primer"     regulatory      441..488                     /regulatory_class="terminator"                     /note="T7 terminator"     rep_origin      1120..1782                     /note="pBBR1 oriV"     misc_feature    2776..3769                     /note="RP4 mob; oriT"     regulatory      complement(3859..3974)                     /regulatory_class="terminator"                     /note="T4 terminator"     gene            complement(4234..5244)                     /gene="aadA"     CDS             complement(4234..5244)                     /gene="aadA"                     /note="streptomycin resistance"                     /codon_start=1                     /transl_table=11                     /product="aminoglycoside-3:adenyltransferase fusion"                     /protein_id="AAQ64664.1"                     /db_xref="GI:34334141"                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV                     GK"     regulatory      5761..5876                     /regulatory_class="terminator"                     /note="T4 terminator"ORIGIN              1 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac       61 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat      121 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga      181 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga      241 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatggggc      301 atcatcatca tcatcatatc gagggaaggc ctgtcgacgg tatcgataag cttgatatcg      361 aattcctgca gcccggggga tccggctgct aacaaagccc gaaaggaagc tgagttggct      421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg      481 ggttttttgc tgaaaggagg aactatatcc ggatcgccct tcccaacagt tgcgcagcct      541 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt      601 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa      661 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata      721 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca      781 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag      841 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga      901 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa      961 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg     1021 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc     1081 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac     1141 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg     1201 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg     1261 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg     1321 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc     1381 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc     1441 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc     1501 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac     1561 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt     1621 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc     1681 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt     1741 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta     1801 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa     1861 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga     1921 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt     1981 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc     2041 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg     2101 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa     2161 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc     2221 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg     2281 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc     2341 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg     2401 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct     2461 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac     2521 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg     2581 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc     2641 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca     2701 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa     2761 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg     2821 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac     2881 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc     2941 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac     3001 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc     3061 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc     3121 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag     3181 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag     3241 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag     3301 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac     3361 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa     3421 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa     3481 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc     3541 aagaaggccg atcaagccca agagacggcc cgagaccttc gggagcgcct gaagcccgtt     3601 ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag     3661 gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag     3721 cgccagcagg aacgcgggcg cgcacatttc cccgaaaagt gccacctggc ggcgttgtga     3781 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt     3841 ggcggtactt gggtcgatag ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa     3901 taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt     3961 caaaaggtca tccaccggat cagcttagta aagccctcgc tagattttaa tgcggatgtt     4021 gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc     4081 ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat     4141 agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc     4201 gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc     4261 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc     4321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag     4381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct     4441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg     4501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg     4561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt     4621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc     4681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat     4741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga     4801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac     4861 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga     4921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac     4981 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt     5041 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc     5101 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca     5161 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt     5221 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc     5281 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt     5341 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt     5401 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac     5461 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt     5521 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt     5581 cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa     5641 ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg     5701 ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagggga attgatccgg     5761 tggatgacct tttgaatgac ctttaataga ttatattact aattaattgg ggaccctaga     5821 ggtccccttt tttattttaa aaattttttc acaaaacggt ttacaagcat aaagctca//