pBMTB-1

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pBMTB-1

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pBMTB-1

载体基本信息

出品人:	Ybscience
载体名称:	pBMTB-1
质粒类型:	广宿主载体；克隆载体；原核表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	araBAD
载体大小:	5029 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       DQ058724                5029 bp    DNA     circular SYN 08-MAY-2006DEFINITION  Broad host range vector pBMTB-1, complete sequence.ACCESSION   DQ058724VERSION     DQ058724.1  GI:69048156  AUTHORS   Lynch,M.D. and Gill,R.T.  TITLE     Broad host range vectors for stable genomic library construction   PUBMED   16496398  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO            80309, USAFEATURES             Location/Qualifiers     source          1..5029                     /organism="Broad host range vector pBMTB-1"                     /mol_type="other DNA"                     /db_xref="taxon:334956"     regulatory      60..96                     /regulatory_class="terminator"                     /note="SoxR terminator"     gene            complement(104..1123)                     /gene="mob"                     /note="mob gene from pBBR1MCS, GenBank Accession Number                     U02374"     CDS             complement(104..1123)                     /gene="mob"                     /note="required for mobilization"                     /codon_start=1                     /transl_table=11                     /product="Mob"                     /protein_id="AAY99697.1"                     /db_xref="GI:69048157"                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK                     KPLLS"     gene            2117..2779                     /gene="rep"                     /note="rep gene from pBBR1, GenBank Accession Number                     X66730"     CDS             2117..2779                     /gene="rep"                     /note="required for replication"                     /codon_start=1                     /transl_table=11                     /product="Rep"                     /protein_id="AAY99698.1"                     /db_xref="GI:69048158"                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP                     RR"     gene            2876..3736                     /gene="bla"                     /note="beta-lactam resistance"     CDS             2876..3736                     /gene="bla"                     /codon_start=1                     /transl_table=11                     /product="beta-lactamase"                     /protein_id="AAY99699.1"                     /db_xref="GI:69048159"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW"     regulatory      3752..3783                     /regulatory_class="terminator"                     /note="TonB terminator"     gene            complement(3811..4740)                     /gene="araC"     CDS             complement(3811..4740)                     /gene="araC"                     /note="regulator"                     /codon_start=1                     /transl_table=11                     /product="AraC"                     /protein_id="AAY99700.1"                     /db_xref="GI:69048160"                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"     regulatory      4748..5015                     /regulatory_class="promoter"                     /note="pBAD promoter"ORIGIN              1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag     2881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt     2941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt     3001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga     3061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat     3121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga     3181 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag     3241 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg     3301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg     3361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt     3421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg     3481 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc     3541 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg     3601 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac     3661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact     3721 gattaagcat tggtaatgag gatccccctc aagtcaaaag cctccggtcg gaggcttttg     3781 actttctgct atggaggtca ggtatgattt ttatgacaac ttgacggcta catcattcac     3841 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac     3901 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat     3961 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa     4021 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac     4081 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg     4141 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat     4201 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc     4261 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc     4321 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca     4381 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg     4441 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa     4501 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc     4561 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt     4621 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc     4681 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat     4741 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc     4801 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa     4861 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg     4921 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca     4981 actctctact gtttctccat acccgttttt ttgggctaga gaattcgtc