pBMTB-4

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pBMTB-4

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pBMTB-4

载体基本信息

出品人:	Ybscience
载体名称:	pBMTB-4
质粒类型:	广宿主载体；克隆载体；原核表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	araBAD
载体大小:	5363 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	四环素
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       DQ058727                5363 bp    DNA     circular SYN 08-MAY-2006DEFINITION  Broad host range vector pBMTB-4, complete sequence.ACCESSION   DQ058727  AUTHORS   Lynch,M.D. and Gill,R.T.  TITLE     Broad host range vectors for stable genomic library construction  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO            80309, USAFEATURES             Location/Qualifiers     source          1..5363                     /organism="Broad host range vector pBMTB-4"                     /mol_type="other DNA"                     /db_xref="taxon:334959"     regulatory      60..96                     /regulatory_class="terminator"                     /note="SoxR terminator"     gene            complement(104..1123)                     /gene="mob"                     /note="mob gene from pBBR1MCS, GenBank Accession Number                     U02374"     CDS             complement(104..1123)                     /gene="mob"                     /note="required for mobilization"                     /codon_start=1                     /transl_table=11                     /product="Mob"                     /protein_id="AAY99709.1"                     /db_xref="GI:69048280"                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK                     KPLLS"     gene            2117..2779                     /gene="rep"                     /note="rep gene from pBBR1, GenBank Accession Number                     X66730"     CDS             2117..2779                     /gene="rep"                     /note="required for replication"                     /codon_start=1                     /transl_table=11                     /product="Rep"                     /protein_id="AAY99710.1"                     /db_xref="GI:69048281"                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP                     RR"     gene            2876..4066                     /gene="tetA"                     /note="tetracycline resistance gene from pBR322"     CDS             2876..4066                     /gene="tetA"                     /codon_start=1                     /transl_table=11                     /product="tetracycline/H+ antiporter"                     /protein_id="AAY99711.1"                     /db_xref="GI:69048282"                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR                     ATST"     regulatory      4086..4117                     /regulatory_class="terminator"                     /note="TonB terminator"     gene            complement(4145..5074)                     /gene="araC"     CDS             complement(4145..5074)                     /gene="araC"                     /note="regulator"                     /codon_start=1                     /transl_table=11                     /product="AraC"                     /protein_id="AAY99712.1"                     /db_xref="GI:69048283"                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"     regulatory      5082..5349                     /regulatory_class="promoter"                     /note="pBAD promoter"ORIGIN              1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgaa     2881 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg     2941 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc     3001 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt     3061 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg     3121 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg     3181 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga     3241 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt     3301 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc     3361 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca     3421 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc     3481 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat     3541 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg     3601 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc     3661 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc     3721 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat     3781 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc     3841 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc     3901 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc     3961 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct     4021 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagggatccc     4081 cctcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg     4141 atttttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact     4201 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa     4261 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg     4321 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga     4381 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa     4441 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca     4501 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca     4561 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc     4621 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg     4681 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc     4741 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg     4801 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc     4861 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa     4921 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa     4981 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc     5041 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt     5101 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa     5161 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag     5221 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa     5281 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt     5341 ttttttgggc tagagaattc gtc