pMHE3

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pMHE3

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pMHE3

载体基本信息

出品人:	Ybscience
载体名称:	pMHE3
质粒类型:	广宿主载体；细菌表达载体;体外表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	T7/lac
载体大小:	6290 bp
5' 测序引物及序列:	T7 promoter 5’TAATACGACTCACTATAGGG 3’
3' 测序引物及序列:	T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
载体标签:	T7 tag (N ter)； 6XHis tag (C ter)
载体抗性:	链霉素
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       AY299694                6290 bp    DNA     circular SYN 10-FEB-2004DEFINITION  Broad host range expression vector pMHE3, complete sequence.ACCESSION   AY299694VERSION     AY299694.1  GI:34334142  TITLE     Modular broad-host-range expression vectors for single-protein and            protein complex purification  JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)   PUBMED   14766546  AUTHORS   Fodor,B.D.  JOURNAL   Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of            Sciences, Temesvari krt., Szeged, Csongrad 6701, HungaryFEATURES             Location/Qualifiers     source          1..6290                     /organism="Broad host range expression vector pMHE3"                     /mol_type="genomic DNA"                     /db_xref="taxon:244052"     primer_bind     111..129                     /note="T7 promoter primer"     regulatory      111..127                     /regulatory_class="promoter"                     /note="T7 promoter"     misc_feature    128..153                     /note="lac operator"     regulatory      184..190                     /regulatory_class="ribosome_binding_site"     misc_feature    199..231                     /note="T7-tag"     misc_feature    280..297                     /note="His-tag"     primer_bind     complement(350..367)                     /note="T7 terminator primer"     regulatory      363..410                     /regulatory_class="terminator"                     /note="T7 terminator"     rep_origin      1436..2098                     /note="pBBR1 oriV"     misc_feature    3092..4085                     /note="RP4 mob; oriT"     regulatory      complement(4175..4290)                     /regulatory_class="terminator"                     /note="T4 terminator"     gene            complement(4550..5341)                     /gene="aadA"     CDS             complement(4550..5341)                     /gene="aadA"                     /note="streptomycin resistance"                     /codon_start=1                     /transl_table=11                     /product="aminoglycoside-3:adenyltransferase fusion"                     /protein_id="AAQ64665.1"                     /db_xref="GI:34334143"                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"     regulatory      6077..6192                     /regulatory_class="terminator"                     /note="T4 terminator"ORIGIN              1 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc       61 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact      121 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt      181 taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg tcgggatccg      241 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga      301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa      361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga      421 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg      481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt      541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc      601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg      661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga      721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc      781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa      841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatattt tgttaaaatt      901 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg      961 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac     1021 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg     1081 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt     1141 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc     1201 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc     1261 tgataaaaac ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc     1321 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg     1381 gctacagccg atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc     1441 ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg     1501 gctcatcggg catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca     1561 aggctggcag gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg     1621 ggccggtcgg tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat     1681 gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg     1741 acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg     1801 acacggtgcc ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct     1861 tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc     1921 tgaccaccac ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg     1981 ccgtgggttt cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca     2041 cccagtgacc gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc     2101 ttatctccat gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt     2161 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct     2221 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt     2281 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc     2341 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa     2401 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg     2461 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg     2521 cttcgccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca     2581 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca     2641 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat     2701 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag     2761 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc     2821 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg     2881 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag     2941 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag     3001 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga     3061 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa     3121 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga     3181 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac     3241 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt     3301 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga     3361 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga     3421 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt     3481 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca     3541 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg     3601 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg     3661 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc     3721 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc     3781 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg     3841 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga     3901 gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc     3961 cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg     4021 ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca     4081 cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt     4141 gaacgaattg ttaggtggcg gtacttgggt cgatagcttt atgcttgtaa accgttttgt     4201 gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt agtaatataa     4261 tctattaaag gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc cctcgctaga     4321 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc     4381 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa     4441 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg     4501 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga     4561 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg     4621 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct     4681 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt     4741 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc     4801 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat     4861 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat     4921 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga     4981 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat     5041 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct     5101 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg     5161 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc     5221 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct     5281 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca     5341 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata     5401 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact     5461 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc     5521 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct     5581 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc     5641 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg     5701 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg     5761 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc     5821 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag     5881 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt     5941 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca     6001 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc     6061 aggggaattg atccggtgga tgaccttttg aatgaccttt aatagattat attactaatt     6121 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac     6181 aagcataaag ctcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat     6241 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt