pMHE6

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pMHE6

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pMHE6

载体基本信息

出品人:	Ybscience
载体名称:	pMHE6
质粒类型:	广宿主载体；细菌表达载体;体外表达载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	T7/lac
载体大小:	5945 bp
5' 测序引物及序列:	T7 promoter 5’TAATACGACTCACTATAGGG 3’
3' 测序引物及序列:	T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
载体标签:	T7 tag（N-ter）；FLAG-tag、Strep-tag II (C-ter)
载体抗性:	链霉素
克隆菌株:	DH5α 等
宿主细胞（系）:	多个菌属
备注:	--
组成型/诱导型:	诱导型

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       AY303672                5945 bp    DNA     circular SYN 10-FEB-2004DEFINITION  Broad host range expression vector pMHE6, complete sequence.ACCESSION   AY303672VERSION     AY303672.1  GI:34766426KEYWORDS    .SOURCE      Broad host range expression vector pMHE6  ORGANISM  Broad host range expression vector pMHE6            other sequences; artificial sequences; vectors.REFERENCE   1  (bases 1 to 5945)  AUTHORS   Fodor,B.D., Kovacs,A.T., Csaki,R., Hunyadi-Gulyas,E., Klement,E.,            Maroti,G., Meszaros,L.S., Medzihradszky,K.F., Rakhely,G. and            Kovacs,K.L.  TITLE     Modular broad-host-range expression vectors for single-protein and            protein complex purification  JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)   PUBMED   14766546REFERENCE   2  (bases 1 to 5945)  AUTHORS   Fodor,B.D.  TITLE     Direct Submission  JOURNAL   Submitted (22-MAY-2003) Biophysics, BRC Hungarian Academy of            Sciences, Temesvari, Szeged, Csongrad 6701, HungaryFEATURES             Location/Qualifiers     source          1..5945                     /organism="Broad host range expression vector pMHE6"                     /mol_type="other DNA"                     /db_xref="taxon:244947"     primer_bind     94..112                     /note="T7 promoter primer"     regulatory      94..110                     /regulatory_class="promoter"                     /note="T7 promoter"     misc_feature    111..136                     /note="lac operator"     misc_feature    182..214                     /note="T7 tag"     misc_feature    278..301                     /note="FLAG-tag"     misc_feature    287..301                     /note="enterokinase cleaveage site"     misc_feature    305..328                     /note="Strep-tag II"     primer_bind     complement(382..399)                     /note="T7 terminator primer"     regulatory      395..442                     /regulatory_class="terminator"                     /note="T7 terminator"     rep_origin      1074..1736                     /note="pBBR1 oriV"     misc_feature    2730..3723                     /note="RP4 mob, oriT"     regulatory      complement(3813..3928)                     /regulatory_class="terminator"                     /note="T4 terminator"     gene            complement(4188..4979)                     /gene="aadA"     CDS             complement(4188..4979)                     /gene="aadA"                     /note="streptomycin resistance"                     /codon_start=1                     /transl_table=11                     /product="aminoglycoside-3:adenyltransferase fusion"                     /protein_id="AAQ82545.1"                     /db_xref="GI:34766427"                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"     regulatory      5715..5830                     /regulatory_class="terminator"                     /note="T4 terminator"ORIGIN              1 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg       61 gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt      121 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca      181 tatggctagc atgactggtg gacagcaaat gggtcgggat ccgaattcga gctccgtcga      241 caagcttgat atcgaattcc tgcagctcga gggatccgac tacaaggacg acgacgacaa      301 gaactggagc catccgcagt tcgagaagtg aagatccggc tgctaacaaa gcccgaaagg      361 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta      421 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg cccttcccaa      481 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg      541 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc gatgagtggc      601 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc      661 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc      721 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta      781 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag      841 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg      901 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc      961 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc     1021 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg     1081 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc     1141 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag     1201 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg     1261 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc     1321 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac     1381 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac     1441 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg     1501 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg     1561 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc     1621 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc     1681 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt     1741 atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc tgcaactttt     1801 cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca gattttcttt     1861 aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta cccccctttt     1921 ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct ttggtgccca     1981 aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc tccggcaaaa     2041 agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc caaggtggcg     2101 ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg cgggcgggct     2161 tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag     2221 ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag     2281 cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg     2341 ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc     2401 ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca     2461 gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc     2521 cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca     2581 agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg     2641 agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc     2701 cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga     2761 agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga     2821 cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg     2881 atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt     2941 tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac     3001 agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc     3061 gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg     3121 tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga     3181 tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg caacggggca     3241 tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc     3301 caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc     3361 agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg     3421 accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag     3481 cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac cttcgggagc     3541 gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag gccaaggccg     3601 ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa gtccagcgcc     3661 agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa aagtgccacc     3721 tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga     3781 acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac cgttttgtga     3841 aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc     3901 tattaaaggt cattcaaaag gtcatccacc ggatcagctt agtaaagccc tcgctagatt     3961 ttaatgcgga tgttgcgatt acttcgccaa ctattgcgat aacaagaaaa agccagcctt     4021 tcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc     4081 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag     4141 ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattg ttagacatta tttgccgact     4201 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag     4261 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga     4321 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg     4381 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca     4441 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc     4501 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt     4561 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata     4621 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa     4681 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg     4741 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt     4801 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg     4861 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg     4921 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg     4981 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac     5041 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt     5101 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg     5161 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta     5221 cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt     5281 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag     5341 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc     5401 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg     5461 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc     5521 gagcatcgtt tgttcgccca gcttctgtat ggaacgggca tgcggatcag tgagggtttg     5581 caactgcggg tcaaggatct ggatttcgat cacggcacga tcatcgtgcg ggagggcaag     5641 ggctccaagg atcgggcctt gatgttaccc gagagcttgg cacccagcct gcgcgagcag     5701 gggaattgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa     5761 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa     5821 gcataaagct caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac     5881 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga     5941 tgtcg