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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pEarleyGate304
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pEarleyGate304载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pEarleyGate304； pEarleyGate 304 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 无 |
| 载体大小: | 10379 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | AcV5 tag (C ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEarleyGate 304 10379 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible promoterless plant transformation vector with a C-terminal AcV5 tag.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 10379) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 10379) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 6487.COMMENT VNTDATE|660096000|COMMENT VNTDBDATE|776390400|COMMENT VNTNAME|pEarleyGate 304|COMMENT VNTAUTHORNAME|Pikaard Lab|FEATURES Location/Qualifiers misc_feature 3..27 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 113..365 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(375..926) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(932..1312) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" protein_bind 1364..1483 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1513..1543 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1597..2256 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 2598..2903 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(2944..3068) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3070..3096 /codon_start=1 /product="epitope tag from the baculovirus Autographa californica GP64 envelope fusion protein" /label=AcV5 tag terminator 3150..3857 /label=OCS terminator /note="octopine synthase terminator" misc_feature 4101..4125 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 5425..6054 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 6483..7556 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 7622..7816 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 8160..8300 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(8486..9074) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9161..9955) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin"ORIGIN 1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg 61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt 121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag 181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg 241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg 301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt 361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg 421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc 481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg 541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg 601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc 661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg 721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg 781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg 841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg 901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa 961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat 1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt 1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta 1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc 1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa 1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga 1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa 1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta 1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag 1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa 2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat 2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag 2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt 2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa 2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga 2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc 2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc 2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc 2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg 2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta 2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc 2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa 3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac 3061 aaagtggtgt cttggaaaga tgcgagcggc tggtcttgac taggtgagtc tagagagtta 3121 attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg 3181 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta 3241 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg 3301 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta 3361 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag 3421 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg 3481 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc 3541 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac 3601 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc 3661 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact 3721 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag 3781 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac 3841 atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa 3901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 3961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 4021 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt 4081 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 4141 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 4201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc 4261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt 4321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg 4381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca 4441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg 4501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga 4561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc 4621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac 4681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt 4741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg 4801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc 4861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg 4921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc 4981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc 5041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga 5101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt 5161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc 5221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc 5281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg 5341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa 5401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg 5461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat 5521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa 5581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc 5641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg 5701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata 5761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg 5821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt 5881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg 5941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac 6001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat 6061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac 6121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca 6181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca 6241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat 6301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa 6361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta 6421 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg 6481 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc 6541 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac 6601 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc 6661 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt 6721 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg 6781 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag 6841 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc 6901 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg 6961 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc 7021 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca 7081 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg 7141 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt 7201 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg 7261 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct 7321 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct 7381 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga 7441 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga 7501 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta 7561 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc 7621 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca 7681 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag 7741 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc 7801 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta 7861 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg 7921 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc 7981 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat 8041 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 8101 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 8161 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat 8221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8281 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 8341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 8401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 8461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 8941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9121 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta 9181 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct 9241 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat 9301 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt 9361 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct 9421 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc 9481 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc 9541 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga 9601 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg 9661 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa 9721 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc 9781 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt 9841 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc 9901 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt 9961 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac 10021 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc 10081 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg 10141 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat 10201 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg 10261 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg 10321 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg//
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