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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pEarleyGate302
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pEarleyGate302载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pEarleyGate302； pEarleyGate 302 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 无 |
| 载体大小: | 10376 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | FLAG (C ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEarleyGate 302 10376 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible promoterless plant transformation vector with a C-terminal FLAG tag.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 10376) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 10376) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 6484.COMMENT VNTDATE|660096000|COMMENT VNTDBDATE|776390400|COMMENT VNTNAME|pEarleyGate 302|COMMENT VNTAUTHORNAME|Pikaard Lab|FEATURES Location/Qualifiers misc_feature 3..27 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 113..365 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(375..926) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(932..1312) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" protein_bind 1364..1483 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1513..1543 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1597..2256 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 2598..2903 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(2944..3068) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3070..3093 /codon_start=1 /product="FLAG epitope tag, followed by an enterokinase cleavage site" /label=FLAG terminator 3147..3854 /label=OCS terminator /note="octopine synthase terminator" misc_feature 4098..4122 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 5422..6051 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 6480..7553 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 7619..7813 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 8157..8297 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(8483..9071) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9158..9952) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin"ORIGIN 1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg 61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt 121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag 181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg 241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg 301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt 361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg 421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc 481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg 541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg 601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc 661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg 721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg 781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg 841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg 901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa 961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat 1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt 1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta 1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc 1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa 1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga 1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa 1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta 1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag 1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa 2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat 2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag 2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt 2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa 2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga 2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc 2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc 2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc 2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg 2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta 2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc 2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa 3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac 3061 aaagtggtgg actacaaaga cgatgacgac aaatgactag gtgagtctag agagttaatt 3121 aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 3181 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 3241 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 3301 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 3361 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 3421 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 3481 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 3541 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 3601 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3661 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3721 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3781 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3841 tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt 3901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 3961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 4021 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag 4081 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt 4141 tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 4201 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct 4261 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc 4321 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg 4381 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga 4441 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact 4501 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga 4561 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg 4621 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg 4681 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg 4741 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt 4801 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag 4861 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc 4921 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct 4981 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc 5041 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg 5101 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat 5161 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca 5221 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc 5281 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt 5341 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa 5401 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca 5461 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt 5521 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat 5581 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc 5641 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc 5701 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg 5761 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta 5821 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc 5881 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc 5941 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct 6001 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag 6061 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca 6121 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact 6181 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga 6241 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa 6301 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca 6361 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc 6421 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg 6481 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 6541 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 6601 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 6661 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 6721 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 6781 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 6841 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 6901 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 6961 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 7021 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 7081 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 7141 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 7201 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 7261 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 7321 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 7381 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 7441 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 7501 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 7561 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg 7621 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 7681 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 7741 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 7801 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc 7861 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 7921 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 7981 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 8041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 8101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 8161 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 8221 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 8281 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8881 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9121 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa 9181 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga 9241 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc 9301 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca 9361 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt 9421 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt 9481 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc 9541 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc 9601 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc 9661 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt 9721 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt 9781 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat 9841 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc 9901 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt 9961 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa 10021 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa 10081 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg 10141 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat 10201 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc 10261 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct 10321 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg//
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