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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pEarleyGate303
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pEarleyGate303载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pEarleyGate303； pEarleyGate 303 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 无 |
| 载体大小: | 10382 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | Myc (C ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEarleyGate 303 10382 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible promoterless plant transformation vector with a C-terminal Myc tag.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 10382) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 10382) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 6490.COMMENT VNTDATE|660096000|COMMENT VNTDBDATE|776390400|COMMENT VNTNAME|pEarleyGate 303|COMMENT VNTAUTHORNAME|Pikaard Lab|FEATURES Location/Qualifiers misc_feature 3..27 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 113..365 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(375..926) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(932..1312) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" protein_bind 1364..1483 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1513..1543 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1597..2256 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 2598..2903 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(2944..3068) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3070..3099 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /label=Myc terminator 3153..3860 /label=OCS terminator /note="octopine synthase terminator" misc_feature 4104..4128 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 5428..6057 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 6486..7559 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 7625..7819 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 8163..8303 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(8489..9077) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9164..9958) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin"ORIGIN 1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg 61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt 121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag 181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg 241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg 301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt 361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg 421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc 481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg 541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg 601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc 661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg 721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg 781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg 841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg 901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa 961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat 1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt 1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta 1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc 1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa 1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga 1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa 1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta 1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag 1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa 2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat 2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag 2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt 2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa 2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga 2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc 2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc 2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc 2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg 2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta 2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc 2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa 3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac 3061 aaagtggtgg aacagaaact gatctctgaa gaagatctgt gactaggtga gtctagagag 3121 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct 3181 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt 3241 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta 3301 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat 3361 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga 3421 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag 3481 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt 3541 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg 3601 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc 3661 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg 3721 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg 3781 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac 3841 aacatgtcga ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta 3901 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 3961 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 4021 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc 4081 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag 4141 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat 4201 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc 4261 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 4321 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 4381 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 4441 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 4501 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 4561 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 4621 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 4681 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 4741 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 4801 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 4861 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 4921 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 4981 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 5041 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 5101 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 5161 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 5221 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 5281 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 5341 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 5401 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 5461 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 5521 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 5581 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 5641 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 5701 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 5761 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 5821 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 5881 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 5941 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 6001 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 6061 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 6121 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 6181 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 6241 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 6301 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 6361 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 6421 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 6481 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg 6541 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag 6601 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac 6661 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt 6721 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg 6781 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc 6841 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg 6901 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga 6961 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc 7021 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca 7081 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat 7141 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg 7201 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc 7261 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc 7321 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga 7381 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc 7441 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc 7501 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat 7561 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct 7621 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt 7681 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa 7741 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa 7801 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc 7861 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg 7921 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg 7981 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt 8041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 8101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 8161 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 8221 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 8281 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 8341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 8401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 8461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 8521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 8581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 8641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 8701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 8761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 8821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 8881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 8941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 9001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 9061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 9121 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca 9181 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata 9241 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt 9301 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat 9361 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg 9421 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt 9481 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta 9541 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc 9601 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt 9661 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt 9721 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt 9781 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt 9841 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta 9901 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt 9961 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa 10021 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt 10081 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc 10141 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga 10201 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac 10261 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg 10321 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct 10381 gg
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