pENTR4

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pENTR4

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pENTR4

载体基本信息

出品公司:	Ybscience
载体名称:	pENTR4
质粒类型:	Gateway载体；入门载体
高拷贝/低拷贝:	高拷贝
启动子:	无
克隆方法:	限制性内切酶；多克隆位点
载体大小:	2720 bp
5' 测序引物及序列:	pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列:	pENTR-R: ATGGCTCATAACACCCCTTG
载体标签:	无
载体抗性:	卡那霉素
真核筛选标记:	无
备注:	pENTR4载体本身含有CCDb致死基因，能够使得普通的DH5alpha等细菌死亡，必须使用DB3.1感受态细胞进行质粒扩增； 构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       Exported                2720 bp ds-DNA     circular SYN 13-AUG-2016DEFINITION  synthetic circular DNAKEYWORDS    pENTR4SOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 2720)  AUTHORS   Biofeng Lab.  TITLE     Direct SubmissionFEATURES             Location/Qualifiers     source          1..2720                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     terminator      103..189                     /gene="Escherichia coli rrnB"                     /note="rrnB T1 terminator"                     /note="transcription terminator T1 from the E. coli rrnB                      gene"     terminator      281..308                     /note="rrnB T2 terminator"                     /note="transcription terminator T2 from the E. coli rrnB                      gene"     protein_bind    358..457                     /gene="mutant version of attL"                     /bound_moiety="LR Clonase(TM)"                     /note="attL1"                     /note="recombination site for the Gateway(R) LR reaction"     CDS             615..920                     /codon_start=1                     /gene="ccdB"                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"                     /note="ccdB"                     /note="Plasmids containing the ccdB gene cannot be                      propagated in standard E. coli strains."                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"     protein_bind    complement(949..1048)                     /gene="mutant version of attL"                     /bound_moiety="LR Clonase(TM)"                     /note="attL2"                     /note="recombination site for the Gateway(R) LR reaction"     CDS             1171..1980                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"     rep_origin      2070..2658                     /direction=RIGHT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"ORIGIN        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc      481 gactggatcc ggtaccgaat tcgcttacta aaagccagat aacagtatgc gtatttgcgc      541 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga      601 ggtgtgcttc tagaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc      661 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc      721 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata      781 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta      841 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc      901 tgatgttctg gggaatatag aattcgcggc cgcactcgag atatctagac ccagctttct      961 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca     1021 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa     1081 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct     1141 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg     1201 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat     1261 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg     1321 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta     1381 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctggt     1441 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa     1501 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat     1561 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg     1621 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc     1681 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca     1741 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata     1801 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta     1861 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt     1921 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa     1981 tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca     2041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     2101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     2161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt     2221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     2281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     2341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     2401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     2461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     2521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     2581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     2641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     2701 tggccttttg ctcacatgtt