pDONR-P4P1R

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pDONR-P4P1R

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pDONR-P4P1R

载体基本信息

出品公司:	Ybscience
载体名称:	pDONR-P4P1R 、pDONR P4-P1R
质粒类型:	Gateway载体；DONR载体
高拷贝/低拷贝:	低拷贝
启动子:	无
克隆方法:	Gateway；BP反应
载体大小:	4777 bp
5' 测序引物及序列:	M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3'
3' 测序引物及序列:	M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3'
载体标签:	无
载体抗性:	卡那霉素，氯霉素（仅空载体）
真核筛选标记:	无
克隆菌株:	阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1
备注:	pDONR-P4P1R载体是MultiSite Gateway载体， 与pDONR221、pDONR-P2RP3载体一同构成三片段系统， 目标载体是pDEST-R4R3，是研究启动子的理想工具。
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported                4779 bp ds-DNA     circular SYN 10-AUG-2016DEFINITION  synthetic circular DNAKEYWORDS    pDONR-P4P1R  AUTHORS   Biofeng Lab.FEATURES             Location/Qualifiers     source          1..4779                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     terminator      268..295                     /note="rrnB T2 terminator"                     /note="transcription terminator T2 from the E. coli rrnB                      gene"     terminator      387..473                     /gene="Escherichia coli rrnB"                     /note="rrnB T1 terminator"                     /note="transcription terminator T1 from the E. coli rrnB                      gene"     primer_bind     537..553                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"     protein_bind    593..824                     /gene="mutant version of attP"                     /bound_moiety="BP Clonase(TM)"                     /note="attP4"                     /note="recombination site for the Gateway(R) BP reaction"     CDS             complement(1181..1486)                     /codon_start=1                     /gene="ccdB"                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"                     /note="ccdB"                     /note="Plasmids containing the ccdB gene cannot be                      propagated in standard E. coli strains."                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"     CDS             complement(1836..2489)                     /codon_start=1                     /gene="cat"                     /product="chloramphenicol acetyltransferase"                     /note="CmR"                     /note="confers resistance to chloramphenicol"                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"     promoter        complement(2490..2592)                     /note="cat promoter"                     /note="promoter of the E. coli cat gene encoding                      chloramphenicol acetyltransferase"     protein_bind    2750..2981                     /gene="mutant version of attP"                     /bound_moiety="BP Clonase(TM)"                     /note="attP1"                     /note="recombination site for the Gateway(R) BP reaction                      (pDONR(TM)201 version)"     promoter        complement(3021..3039)                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     primer_bind     complement(3044..3060)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"     CDS             3173..3982                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"     rep_origin      4129..4717                     /direction=RIGHT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"ORIGIN        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt      661 ataatgccaa ctttgtatag aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat      721 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatg      781 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagaattc gagctcgacc      841 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt      901 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt      961 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc     1021 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc     1081 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt     1141 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca     1201 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga     1261 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga     1321 tatgcaccac cgggtaaagt tcacgggaga ctttatccga cagcagacgt gcactggcca     1381 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca     1441 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg     1501 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc cgtcccttcc     1561 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg     1621 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt     1681 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata     1741 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct     1801 ggcttttagt aagccggatc cacgcggcgt ttacgccccc cctgccactc atcgcagtac     1861 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc     1921 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa     1981 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc     2041 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg     2101 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg     2161 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa     2221 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga     2281 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt     2341 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat     2401 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg     2461 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat     2521 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt     2581 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca     2641 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg     2701 caaagtgcgt cgggtgatgc tgccaactta gtcgacgctc tagagctcca aataatgatt     2761 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata     2821 atgccaactt tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata     2881 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag     2941 tcactatgaa ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct     3001 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg     3061 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc     3121 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca     3181 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg     3241 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg     3301 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt     3361 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa     3421 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac     3481 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc     3541 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg     3601 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga     3661 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact     3721 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat     3781 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg     3841 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa     3901 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt     3961 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat     4021 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc     4081 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt     4141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg     4201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca     4261 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt     4321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga     4381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc     4441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact     4501 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga     4561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg     4621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt     4681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt     4741 acggttcctg gccttttgct ggccttttgc tcacatgtt