pDONR221-P4RP3R

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pDONR221-P4RP3R

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pDONR221-P4RP3R

载体基本信息

出品公司:	Ybscience
载体名称:	pDONR221-P4RP3R
质粒类型:	Mltisite-Gateway载体；DONR载体
高拷贝/低拷贝:	低拷贝
启动子:	无
克隆方法:	Gateway；BP反应
载体大小:	4717 bp
5' 测序引物及序列:	M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3'
3' 测序引物及序列:	M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3'
载体标签:	无
载体抗性:	卡那霉素，氯霉素（仅空载体）
真核筛选标记:	无
克隆菌株:	阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1
备注:	pDONR221-P5P2载体是MultiSite Gateway载体， 目标载体如 pAd/PL-DEST、pcDNA6/V5-PL-DEST载体，是研究启动子的理想工具。
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported                4717 bp ds-DNA     circular SYN 10-AUG-2016KEYWORDS    pDONR221-P4rP3rSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4717)  AUTHORS   Biofeng Lab.  TITLE     Direct SubmissionFEATURES             Location/Qualifiers     source          1..4717                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     terminator      268..295                     /note="rrnB T2 terminator"                     /note="transcription terminator T2 from the E. coli rrnB                      gene"     terminator      387..473                     /gene="Escherichia coli rrnB"                     /note="rrnB T1 terminator"                     /note="transcription terminator T1 from the E. coli rrnB                      gene"     primer_bind     537..553                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"     protein_bind    complement(570..801)                     /gene="mutant version of attP"                     /bound_moiety="BP Clonase(TM)"                     /note="attP4"                     /note="recombination site for the Gateway(R) BP reaction"     CDS             complement(1149..1454)                     /codon_start=1                     /gene="ccdB"                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"                     /note="ccdB"                     /note="Plasmids containing the ccdB gene cannot be                      propagated in standard E. coli strains."                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"     CDS             complement(1804..2457)                     /codon_start=1                     /gene="cat"                     /product="chloramphenicol acetyltransferase"                     /note="CmR"                     /note="confers resistance to chloramphenicol"                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"     promoter        complement(2458..2560)                     /note="cat promoter"                     /note="promoter of the E. coli cat gene encoding                      chloramphenicol acetyltransferase"     protein_bind    2705..2936                     /gene="mutant version of attP"                     /bound_moiety="BP Clonase(TM)"                     /note="attP3"                     /note="recombination site for the Gateway(R) BP reaction"     promoter        complement(2959..2977)                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     primer_bind     complement(2982..2998)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"     CDS             3111..3920                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"     rep_origin      4067..4655                     /direction=RIGHT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"ORIGIN        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca      721 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca      781 gtcaaaataa aatcattatt tgtcgaccga cagccttcca aatgttcttc tcaaacggaa      841 tcgtcgtatc cagcctactc gctattgtcc tcaatgccgt attaaatcat aaaaagaaat      901 aagaaaaaga ggtgcgagcc tcttttttgt gtgacaaaat aaaaacatct acctattcat      961 atacgctagt gtcatagtcc tgaaaatcat ctgcatcaag aacaatttca caactcttat     1021 acttttctct tacaagtcgt tcggcttcat ctggattttc agcctctata cttactaaac     1081 gtgataaagt ttctgtaatt tctactgtat cgacctgcag actggctgtg tataagggag     1141 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg     1201 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg     1261 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact     1321 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca     1381 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa     1441 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat     1501 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag     1561 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat     1621 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca     1681 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat     1741 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca cgcggcgttt     1801 acgccccccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg     1861 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc     1921 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac     1981 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc     2041 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata     2101 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc     2161 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc     2221 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat     2281 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc     2341 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc     2401 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt     2461 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat     2521 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa     2581 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg     2641 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttagt     2701 cgacaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag     2761 caatgctttt ttataatgcc aactttgtat aataaagttg aacgagaaac gtaaaatgat     2821 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa     2881 acacaacata tccagtcact atgaatcaac tacttagatg gtattagtga cctgtactgc     2941 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc     3001 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat     3061 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata     3121 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt     3181 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga     3241 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta     3301 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc     3361 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag     3421 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag     3481 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg     3541 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt     3601 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt     3661 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt     3721 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat     3781 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac     3841 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga     3901 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta     3961 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt     4021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     4081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     4141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     4201 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     4261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     4321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     4381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     4441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     4501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     4561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     4621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     4681 ggttcctggc cttttgctgg ccttttgctc acatgtt