pFB

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pFB

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pFB

载体基本信息

出品公司:	Ybscience
载体名称:	pFB
质粒类型:	逆病毒载体
高拷贝/低拷贝:	低拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	--
载体大小:	5117 bp
5' 测序引物及序列:	CMV Forward： CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	无
克隆菌株:	DH5α 等通用菌株
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	逆转录病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pFB载体描述The Agilent pFB and pFB-Neo plasmid vectors for retroviral gene delivery and expression are derived from the Moloney murine leukemia virus (MMLV) and can be used to produce high titer viral stocks with MMLVbased packaging cell lines.1,2 Both vectors contain the bacterial origin of replication and ampicillin-resistance gene from pBR322, an extended MMLV packaging signal (ψ+), and a multiple cloning site (MCS) located between the MMLV 5′ and 3′ long terminal repeat sequences (LTRs).The pFB vector is a minimal MMLV-based vector that can accommodate larger inserts than pFB-Neo. The pFB vector does not contain any exogenous sequence other than the sequence of the MCS.The pFB-Neo vector differs from the pFB vector only by the presence of a cassette consisting of the internal ribosome entry site (IRES) from the encephalomyocarditis virus (EMCV) and the neomycin-resistance gene (neor). The open reading frame (ORF) for the neor gene is positioned downstream of the MCS and follows the IRES. A dicistronic message encoding the gene of interest and the neor gene is expressed from the viral promoter within the 5′ LTR.pFB载体限制性酶切位点pFB, 5117 bp			             version 013001Enzymes with 1-10 cleavage sites:                       #sites   --  Bp position of recognition site --  AatII          2      978,   5043  Acc65I         3      647,   1846,   2643  AccI           3     1967,   2057,   3003  AflII          3      202,   1265,   2198  AflIII         2      164,   3232  AgeI           1     1840  AhdI           6      687,    733,   1274,   2683,   2729                       4120  Alw44I         4     1054,   3048,   3546,   4792  AlwNI          5      323,    398,   2319,   2394,   3643  ApaI           1     1238  ApoI           3       87,   1128,   2063  AvaI           9      577,    610,    643,   1241,   2018                       2074,   2573,   2606,   2639  BamHI          1     2069  BanII          7      580,    593,    764,   1238,   2576                       2589,   2760  BbeI           3      615,   1656,   2611  BbsI           1     5110  BbvCI          2      453,   2449  BciVI          5      656,   1986,   2652,   3441,   4968  BglI           1     4239  BglII          1     1678  BpmI           3     1799,   2146,   4210  Bpu10I         7      337,    412,    453,   1548,   2333                       2408,   2449  BsaAI          3     1816,   2000,   2984  BsaHI          6      615,    978,   1656,   2611,   4661                       5043  BsaI           9      694,    715,    782,   1414,   1802                       2690,   2711,   2778,   4192  BsaWI          5      138,   1840,   3438,   3585,   4416  BseRI          5      718,   1068,   1561,   1600,   2714  BsiEI          8      961,   1034,   1838,   2080,   3145                       3569,   4492,   4641  BsiHKAI        7      580,   1054,   2576,   3048,   3546                       4707,   4792  BsmBI          6      976,   1093,   1337,   1396,   1582                       2881  Bsp120I        1     1238  BspHI          3     3952,   4960,   5065  BspLU11I       2      164,   3232  BsrBI          5     1299,   2077,   2102,   3163,   4964  BsrDI          2     4179,   4361  BsrGI          1     1541  BssHII         2      563,   2559  BssSI          3     3405,   4789,   5096  Bst1107I       1     3003  BstAPI         5      322,    397,   2318,   2393,   3050  BstEII         1     1346  BstF5I         9      682,   1386,   1573,   2031,   2678                       2907,   4105,   4286,   4573  Bsu36I         1     1276  Cfr10I         2     1840,   4205  Csp6I          8      648,    895,   1542,   1819,   1847                       2644,   3039,   4604  DraI           3     3989,   4008,   4700  DraIII         1     1873  DrdI           2     2921,   3334  DsaI           2      151,    931  EaeI           7      827,    961,   1368,   1389,   1668                       2080,   4513  EarI           4     1334,   1564,   3110,   4914  Ecl136II       2      580,   2576  Eco52I         2      961,   2080  Eco57I         2     3759,   4807  Eco72I         1     2000  EcoNI          1     1647  EcoO109I       5      499,   1477,   1924,   2495,   5100  EcoRI          1     2063  EcoRV          4      308,    383,   2304,   2379  EheI           3      615,   1656,   2611  FokI           9      682,   1386,   1573,   2031,   2678                       2907,   4105,   4286,   4573  FspI           1     4345  HaeII          5      615,   1656,   2611,   3106,   3476  HgaI           6      889,   1166,   2938,   3334,   3912                       4662  HincII         2     2057,   4664  HinfI         10      637,   1020,   1045,   1863,   2108                       2633,   3132,   3207,   3603,   4120  KasI           3      615,   1656,   2611  KpnI           3      647,   1846,   2643  MaeII         10      979,   1340,   1381,   1817,   2001                       2985,   3935,   4351,   4724,   5044  MboII         10     1334,   1565,   2096,   3111,   3882                       3973,   4728,   4806,   4915,   5111  MscI           3      827,   1368,   1668  MslI           4      159,   4373,   4532,   4891  MunI           2       11,     21  NarI           3      615,   1656,   2611  NdeI           3     1664,   1672,   3054  NheI           5        6,     16,     26,    197,   2193  NotI           1     2079  NspI           3      164,   2867,   3232  PleI           9      637,   1020,   1045,   1863,   2108                       2633,   3132,   3603,   4120  PpuMI          4      499,   1477,   1924,   2495  PshAI          1     1015  Psp1406I       2     4350,   4723  PstI           3     1178,   1360,   4366  PvuI           2     1034,   4492  PvuII          4      286,    361,   2282,   2357  RsaI           8      648,    895,   1542,   1819,   1847                       2644,   3039,   4604  SacI           2      580,   2576  SacII          1      151  SalI           1     2057  SapI           1     3109  ScaI           1     4603  SchI           9      637,   1020,   1045,   1863,   2108                       2633,   3132,   3603,   4120  SexAI          1     1474  SfcI           7      182,   1178,   1360,   2178,   3497                       3688,   4366  SmaI           4      643,   1241,   2018,   2639  SmlI           8      202,   1265,   2074,   2198,   3338                       3600,   3877,   4745  SpeI           1      897  SrfI           1     1240  SspI           1     4927  StyI           5      496,    706,   1504,   2492,   2702  TaiI          10      979,   1340,   1381,   1817,   2001                       2985,   3935,   4351,   4724,   5044  TaqI           9     1297,   1608,   1619,   2050,   2058                       2075,   2119,   3332,   4776  TatI           3     1541,   3038,   4603  TfiI           1     3207  Tsp45I         6     1282,   1491,   2887,   2982,   4382                       4593  TspRI         10     1284,   1875,   3128,   3634,   3647                       3918,   4067,   4172,   4519,   4546  Tth111I        4      633,   1473,   2629,   2976  VspI           1     4296  XbaI           3      464,   2036,   2460  XhoI           1     2074  XhoII          8     1678,   2069,   3873,   3884,   3970                       3982,   4750,   4767  XmaI           4      643,   1241,   2018,   2639  XmnI           1     4720Enzymes that do NOT cut molecule:AccIII      AscI        BclI        BlnI        Bpu1102I    BsaBI       BsgI        BsiWI       BsmI        BspMI       BstBI       BstXI       ClaI        Eco47III    FseI        HindIII     HpaI        MluI        NaeI        NcoI        NgoMIV      NruI        NsiI        PacI        PmeI        Ppu10I      RsrII       SanDI       SbfI        SfiI        SgfI        SgrAI       SnaBI       SphI        StuI        SwaI        Van91I      XcmI        

 

载体序列

pFB, 5117 bp                                  version 013001NOTE: The following sequence has been verified for accuracyat the junctions. The remainder of the sequence has been obtained from existing data.    1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG    51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT   101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG   151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA   201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT   251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC   301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA   351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG   401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA   451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG   501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC   551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC   601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA   651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG   701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG   751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC   801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG   851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA   901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT   951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC  1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG  1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA  1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC  1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG  1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA  1301  GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA  1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC  1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC  1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG  1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT  1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC  1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT  1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG  1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC  1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT  1801  GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC  1851  CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT  1901  AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC  1951  CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC  2001  ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT  2051  CGAATTGTCG ACGAATTCGG ATCCTCGAGC GGCCGCGATC CGAGTTCTTC  2101  TGAGCGGGAC TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA  2151  GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT  2201  AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAG  2251  AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA  2301  CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG  2351  ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC  2401  TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC  2451  TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC  2501  TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC  2551  TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC  2601  CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG  2651  TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT  2701  TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT  2751  TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC  2801  GACCCACCAC CGGGAGGTAA GCTGGCTGCC TCGCGCGTTT CGGTGATGAC  2851  GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT  2901  GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG  2951  TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA  3001  GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG  3051  CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG  3101  CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG  3151  TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA  3201  TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA  3251  GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA  3301  GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG  3351  TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG  3401  CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT  3451  CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT  3501  AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA  3551  CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC  3601  TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT  3651  GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT  3701  GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT  3751  GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA  3801  TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA  3851  GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT  3901  CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG  3951  GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA  4001  ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA  4051  GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT  4101  CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG  4151  GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC  4201  GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC  4251  GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA  4301  TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA  4351  ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT  4401  ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC  4451  CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG  4501  TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG  4551  CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG  4601  TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT  4651  GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT  4701  TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG  4751  GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA  4801  ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA  4851  ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG  4901  TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG  4951  GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA  5001  CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA  5051  AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA  5101  GGCCCTTTCG TCTTCAA