pBABE

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pBABE

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pBABE

载体基本信息

出品人:	Ybscience
载体名称:	pBABE
质粒类型:	逆病毒载体；荧光报告载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	MSCV
载体大小:	5316 bp
5' 测序引物及序列:	pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列:	pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:	无
载体抗性:	氨苄青霉素
筛选标记:	EGFP
克隆菌株:	DH5α、HB101等
宿主细胞（系）:	常规哺乳动物细胞系
备注:	推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	逆转录病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pBABE	5316 bp 	DNA	SYNDEFINITION  pBABEACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..5316                     /organism="pBABE"                     /mol_type="other DNA"     misc_feature    11..460                     /label="3MoMuLV_LTR"     misc_feature    17..460                     /label="5_LTR2"     misc_feature    530..1339                     /label="psi_plus_pack"     misc_feature    926..1333                     /label="gag"     misc_feature    1269..1291                     /label="pLXSN_5_primer"     misc_feature    1269..1291                     /label="MSCV_primer"     misc_feature    1307..1323                     /label="pBABE_5_primer"     misc_feature    complement(1396..1416)                     /label="pBABE_3_primer"     misc_feature    complement(1402..1617)                     /label="SV40_enhancer"     promoter        1414..1682                     /label="SV40_promoter"     rep_origin      1581..1658                     /label="SV40_origin"     misc_feature    1643..1662                     /label="SV40pro_F_primer"     CDS             1764..2621                     /label="ORF frame 3"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     gene            1767..2480                     /label="EGFP"                     /gene="EGFP"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     misc_feature    complement(1809..1830)                     /label="EGFP_N_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     misc_feature    1947..1976                     /label="Y66 (EGFP)"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     misc_feature    2417..2438                     /label="EGFP_C_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     misc_feature    2711..3250                     /label="5_LTR2"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     gene            complement(4309..5169)                     /label="Ampicillin"                     /gene="Ampicillin"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYKYSDPLRESWTTKVNWMAHTVRKDYRSKLNPPDESGPG                     CMSCKCVLS*"     CDS             complement(4309..5169)                     /label="ORF frame 1"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(5211..5239)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    5309..460                     /label="5_LTR2"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    5315..460                     /label="3MoMuLV_LTR"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA   61 CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC  121 CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA  181 TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC  241 AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC  301 AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC  361 CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC  421 CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG  481 GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA  541 TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA  601 GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC  661 CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG  721 AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA  781 GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG  841 CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT  901 TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG  961 TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG 1021 TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG 1081 CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA 1141 TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC 1201 TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC 1261 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT 1321 CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACGTAGAA TTCCCAGCAC 1381 AGTGGTCGAC CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 1441 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 1501 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 1561 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 1621 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC 1681 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTGGCCG 1741 CTCTAGCGCT ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG 1801 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG 1861 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 1921 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA 1981 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT 2041 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG 2101 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG 2161 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA 2221 TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG 2281 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC 2341 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA 2401 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG 2461 GCATGGACGA GCTGTACAAG TACTCAGATC CGCTCAGGGA AAGCTGGACT ACTAAAGTGA 2521 ACTGGATGGC CCACACAGTT CGGAAAGACT ACAGATCTAA GCTGAACCCT CCTGATGAGA 2581 GTGGCCCCGG CTGCATGAGC TGCAAGTGTG TGCTCTCCTG AGGATCGATA AAATAAAAGA 2641 TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC 2701 TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT 2761 TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG 2821 TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA 2881 ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC 2941 AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA 3001 AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT 3061 GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG 3121 CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG 3181 TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC 3241 CCGTCAGCGG GGGTCTTTCA CATGCAGCAT GTATCAAAAT TAATTTGGTT TTTTTTCTTA 3301 AGTATTTACA TTAAATGGCC ATAGTTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 3361 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 3421 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 3481 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 3541 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 3601 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 3661 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 3721 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 3781 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 3841 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 3901 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 3961 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 4021 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 4081 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 4141 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 4201 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTG 4261 CGGCCGCAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 4321 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 4381 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 4441 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 4501 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 4561 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 4621 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 4681 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 4741 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 4801 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 4861 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 4921 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 4981 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 5041 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 5101 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 5161 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 5221 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 5281 TCCCCGAAAA GTGCCACCTG CAGCCTGAAT ATGGGC