pGIPZ

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pGIPZ

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pGIPZ

载体基本信息

出品公司:	Ybscience
载体名称:	pGIPZ
质粒类型:	慢病毒系统载体
高拷贝/低拷贝:	--
启动子:	CMV
克隆方法:	多克隆位点，限制性内切酶
载体大小:	11688bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素（Ampicillian）
筛选标记:	嘌呤霉素（Puromycin）
备注:	应与二代包装质粒psPAX2一同使用。
稳定性:	--
组成型:	--
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pGIPZ	11688 bp 	DNA	SYNDEFINITION  pGIPZACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..11688                     /organism="pGIPZ"                     /mol_type="other DNA"     misc_feature    454..634                     /label="HIV-1_5_LTR"     misc_feature    454..634                     /label="truncHIV-1_3_LTR"     misc_feature    745..789                     /label="HIV-1_psi_pack"     CDS             complement(1015..1476)                     /label="ORF frame 1"     CDS             1181..2068                     /label="ORF frame 2"     misc_feature    1303..1536                     /label="RRE"     misc_feature    2067..2082                     /label="cPPT"     misc_feature    complement(2245..2619)                     /label="sh_ble"     gene            complement(2248..2619)                     /label="bleo"                     /gene="bleo"     promoter        complement(2629..2647)                     /label="T7_promoter"     promoter        2740..3292                     /label="CMV_immearly_promoter"     misc_feature    2795..3082                     /label="CAG_enhancer"     misc_feature    3249..3269                     /label="CMV_fwd_primer"       promoter        3250..3319                     /label="CMV_promoter"     misc_feature    3293..3312                     /label="pCEP_fwd_primer"     misc_feature    3295..3319                     /label="LNCX_primer"     CDS             3390..4088                     /label="ORF frame 3"     misc_feature    4114..4677                     /label="IRES"     CDS             4690..5292                     /label="ORF frame 1"     gene            4705..5292                     /label="puro (variant)"                     /gene="puro (variant)"     CDS             5606..6280                     /label="ORF frame 2"     promoter        complement(5625..5643)                     /label="T7_promoter"     misc_feature    5665..6240                     /label="WPRE"     misc_feature    6458..6510                     /label="delta_U3"     misc_feature    6511..6691                     /label="HIV-1_5_LTR"     misc_feature    6511..6691                     /label="truncHIV-1_3_LTR"     misc_feature    complement(6792..6809)                     /label="BGH_rev_primer"     terminator      6795..7022                     /label="bGH_PA_terminator"     rep_origin      7085..7391                     /label="f1_origin"     misc_feature    complement(7505..7525)                     /label="pBABE_3_primer"     misc_feature    complement(7511..7726)                     /label="SV40_enhancer"     promoter        7523..7791                     /label="SV40_promoter"     rep_origin      7690..7767                     /label="SV40_origin"     misc_feature    7752..7771                     /label="SV40pro_F_primer"     CDS             complement(7825..8646)                     /label="ORF frame 1"     CDS             7888..8913                     /label="ORF frame 1"     gene            7903..8910                     /label="hygroB"                     /gene="hygroB"     terminator      9046..9165                     /label="SV40_PA_terminator"     misc_feature    9134..9153                     /label="EBV_rev_primer"     promoter        complement(9209..9227)                     /label="M13_reverse_primer"     misc_feature    complement(9226..9248)                     /label="M13_pUC_rev_primer"     promoter        complement(9262..9291)                     /label="lac_promoter"     gene            complement(10374..11234)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(10374..11234)                     /label="ORF frame 2"     promoter        complement(11276..11304)                     /label="AmpR_promoter"     terminator      11390..11521                     /label="SV40_PA_terminator"     misc_feature    11478..11497                     /label="EBV_rev_primer"ORIGIN    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc 2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg 3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag 3361 acaccgactc tactagagga tctgccacca tggagagcga cgagagcggc ctgcccgcca 3421 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg 3481 gcggagaggg cacccccgag cagggccgca tgaccaacaa gatgaagagc accaaaggcg 3541 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg 3601 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca 3661 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc 3721 gctacgaggc cggccgcgtg atcggcgact tcaaggtgat gggcaccggc ttccccgagg 3781 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc 3841 ccatgggcga taacgatctg gatggcagct tcacccgcac cttcagcctg cgcgacggcg 3901 gctactacag ctccgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca 3961 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggaggat cacagcaaca 4021 ccgagctggg catcgtggag taccagcacg ccttcaagac cccggatgca gatgccggtg 4081 aagaataatg tacaagtagc ggccgcaaat tccgcccctc tccctccccc ccccctaacg 4141 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 4201 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 4261 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 4321 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 4381 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 4441 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 4501 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 4561 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 4621 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 4681 gataatacca tggccaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4741 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4801 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4861 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4921 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggctc gcgcatggcc 4981 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5041 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5101 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgctggggtg 5161 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5221 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5281 cccggtgcct gagtttgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca 5341 tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg ctcgaggtaa 5401 ccggatcctg atcagaattc aaggggctac tttaggagca attatcttgt ttactaaaac 5461 tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt 5521 aaatcacttt tttcaattgg aagactaatg cggccggcca ttactccgtc tcgtgtcttg 5581 ttgcatatgt ctgctggttt gtttgatgtt gtttgcgggc gggccctata gtgagtcgta 5641 ttacctagga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac 5701 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 5761 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 5821 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 5881 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5941 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 6001 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 6061 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 6121 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 6181 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 6241 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca 6301 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 6361 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct 6421 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa 6481 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 6541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 6601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 6661 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 6721 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgtttaaac 6781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 7021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 7081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 7141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 7201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 7261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 7321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 7381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 7441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 7501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 7561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 7621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 7681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 7801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 7861 tccattttcg gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc 7921 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc 7981 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat 8041 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg 8101 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc 8161 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt 8221 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc 8281 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata 8341 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt 8401 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc 8461 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata 8521 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac 8581 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg 8641 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt 8701 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt 8761 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc 8821 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga 8881 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc 8941 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 9001 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 9061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 9181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 9241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 9301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 9361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 9421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 9481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 9541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 9601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 9661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 9721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 9781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 9841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 9901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 9961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc10021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg10081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc10141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct10201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt10261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa10321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa10381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc10441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct10501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca10561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt10621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt10681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc10741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc10801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt10861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact10921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc10981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt11041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg11101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct11161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa11221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt11281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc11341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc11401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt11461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat11521 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac11581 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc11641 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt