pLVX-TetOne-Puro

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pLVX-TetOne-Puro

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pLVX-TetOne-Puro

载体基本信息

出品公司:	Ybscience
载体名称:	pLVX-TetOne-Puro
质粒类型:	慢病毒载体；四环素调控载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	TRE3GS
载体大小:	9227 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素（Puromycin）
克隆菌株:	Stbl3
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	慢病毒载体pLVX-TetOne-Puro是集调控与应答功能于一体的四环素诱导载体。
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pLVX-TetOne-puro           9227 bp DNA   circular 18-JUL-2013COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Catalog Nos. 631847, 631848, 631849FEATURES             Location/Qualifiers     LTR             1..635                     /label="5' LTR"     misc_feature    681..806                     /label="Packaging Signal"     misc_feature    1303..1536                     /label="RRE"     misc_feature    2028..2144                     /label="cPPT/CTS"     polyA_signal    2187..2321                     /label="SV40 polyA signal"     misc_feature    complement(2496..2527)                     /label="MCS"     promoter        complement(2528..2892)                     /label="TRE3Gs promoter"     promoter        2912..3422                     /label="hPGK promoter"     CDS             3441..4187                     /label="Tet-On 3G"     promoter        4198..4527                     /label="SV40 promoter"     CDS             4536..5135                     /label="PuroR"     misc_feature    5149..5737                     /label="WPRE"     LTR             5945..6578                     /label="3' LTR"     rep_origin      complement(7109..7694)                     /label="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(7865..8725)                     /label="AmpR"     promoter        complement(8726..8830)                     /label="AmpR promoter"ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg     2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg     2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca     2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg     2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt     2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa     2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg     2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca     2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg     3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg     3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct     3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg     3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata     3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg     3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc     3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc     3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc     3481 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc     3541 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg     3601 ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt     3661 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc     3721 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg     3781 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc     3841 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag     3901 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa     3961 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca     4021 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg     4081 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc     4141 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg cgcgaatgtg     4201 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     4261 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt     4321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc     4381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt     4441 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc     4501 ttttttggag gcctaggctt ttgcaaaacg cgaccatgac cgagtacaag cccacggtgc     4561 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg     4621 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc     4681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg     4741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg     4801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga     4861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg     4921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg     4981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc     5041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc     5101 gcacctggtg catgacccgc aagcccggtg cctgaacgcg tctggaacaa tcaacctctg     5161 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta     5221 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt     5281 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc     5341 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt     5401 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg     5461 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac     5521 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc     5581 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac     5641 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct     5701 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag     5761 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg     5821 cctggctaga agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct     5881 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg     5941 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac     6001 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat     6061 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag     6121 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac     6181 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc     6241 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt     6301 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc     6361 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat     6421 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt     6481 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc     6541 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat     6601 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg     6661 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt     6781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc     6841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg     6901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     7621 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa     7681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     7741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc     7801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg     7861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat     7921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg     7981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa     8041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca     8101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc     8161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt     8221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa     8281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat     8341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct     8401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga     8461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag     8521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga     8581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca     8641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg     8701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc     8761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     8821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac     8881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat     8941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat     9001 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca     9061 taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc     9121 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt     9181 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt