pLP2

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pLP2

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pLP2

载体基本信息

出品公司:	Ybscience
载体名称:	pLP2
质粒类型:	哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝:	高拷贝
启动子:	RSV
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4180 bp
5' 测序引物及序列:	CMV-F:  CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄
备注:	载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。 第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG（或者pCMV-VSVG）。在使用的时候，按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时，对于60mm的细胞培养皿来说，按照慢病毒基因质粒（3ug）和package mix（6 ug，也就是6 ul）的比例进行慢病毒质粒包装实验。
稳定性:	/
组成型:	--
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported File           4180 bp ds-DNA    circular SYN 10-一月-2013DEFINITION  Lentiviral packaging plasmid containing the HIV-1 rev gene.ACCESSION   .VERSION     .KEYWORDS    pLP2SOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructCOMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..4180                     /organism="synthetic DNA construct"                     /lab_host="M"                     /mol_type="other DNA"     promoter        1..262                     /note="RSV promoter"                     /note="Rous sarcoma virus enhancer/promoter"     CDS             391..741                     /codon_start=1                     /product="HIV-1 Rev protein"                     /note="rev"     polyA_signal    850..971                     /note="HIV-1 poly(A)"                     /note="polyadenylation signal from the LTR of HIV-1"     rep_origin      1173..1628                     /direction=RIGHT                     /note="f1 ori"                     /note="f1 bacteriophage origin of replication; arrow                      indicates direction of (+) strand synthesis"     promoter        1910..2014                     /gene="bla"                     /note="AmpR promoter"     CDS             2015..2875                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     rep_origin      3046..3634                     /direction=RIGHT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"ORIGIN        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc      241 attcaccaca ttggtgtgca cctccaagct cgagctcgtt tagtgaaccg tcagatcgcc      301 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc      361 ccctcgaagc tagtcgatta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa      421 gacctcctca aggcagtcag actcatcaag tttctctatc aaagcaaccc acctcccaat      481 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga      541 cagatccatt cgattagtga acggatcctt agcacttatc tgggacgatc tgcggagcct      601 gtgcctcttc agctaccacc gcttgagaga cttactcttg attgtaacga ggattgtgga      661 acttctggga cgcagggggt gggaagccct caaatattgg tggaatctcc tacaatattg      721 gagtcaggag ctaaagaata gtgctgttag cttgctcaat gccacagcta tagcagtagc      781 tgaggggaca gatagggtta tagaagtagt acaagaagct tggcactggc cgtcgtttta      841 caacgtcgtg atctgagcct gggagatctc tggctaacta gggaacccac tgcttaagcc      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      961 taactagaga tcaggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc     1021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     1081 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta     1141 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc     1201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     1261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     1321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     1381 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     1441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     1501 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg     1561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt     1621 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc     1681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     1741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     1801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat     1861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc     1921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg     1981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc     2041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt     2101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct     2161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac     2221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact     2281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa     2341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga     2401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt     2461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga     2521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg     2581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat     2641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat     2701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc     2761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga     2821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc     2881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag     2941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc     3001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt     3061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt     3121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat     3181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc     3241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa     3301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg     3361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag     3421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag     3481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa     3541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt     3601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg     3661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc     3721 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac     3781 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct     3841 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc     3901 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt     3961 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac     4021 aggaaacagc tatgacatga ttacgaattc gatgtacggg ccagatatac gcgtatctga     4081 ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc     4141 ctcaggatat agtagtttcg cttttgcata gggaggggga